Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content

Yadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. Alice

http://hdl.handle.net/10198/34339

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Resumo(s)

Genetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.

Palavras-chave

Honey bee Spermatheca Genomic DNA isolation DNA extraction

URI

http://hdl.handle.net/10198/34339

Citação

Yadró, Carlos A.; Lopes, Ana Rita; Henriques, Dora; Musin, Eduard; Wegener, Jakob; Pinto, M. Alice (2023). Genomic DNA extraction from honey bee (Apis mellifera) queen spermathecal content. Journal of Apicultural Research. ISSN 0021-8839. 64:1, p. 198-201