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Phytochemical profile and biological activities of 'Ora-pro-nobis' leaves (Pereskia aculeata Miller), an underexploited superfood from the Brazilian Atlantic Forest

2019Conference object Open access
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dc.contributor.authorGarcia, Jéssica A.A.
dc.contributor.authorCorrêa, Rúbia C.G.
dc.contributor.authorBarros, Lillian
dc.contributor.authorPereira, Carla
dc.contributor.authorAbreu, Rui M.V.
dc.contributor.authorAlves, Maria José
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorBracht, Adelar
dc.contributor.authorPeralta, Rosane M.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2021-10-18T13:53:05Z
dc.date.available2021-10-18T13:53:05Z
dc.date.issued2019
dc.description.abstractSouth to Northeast of Brazil, ora-pro-nobis leaves are used in traditional cuisine, featuring as an ingredient of various sweet and savoury dishes. In some low-income communities, it is known as the ‘meat of the poor’, being the main protein source available.1 The aim of the present work was to perform an inedited study on the phytochemical profile and some biological activities of P. aculeate leaves, with the view of expanding the current knowledge on the potentialities of this superfood. For this purpose, its hydroethanolic extract was characterized in terms of phenolic composition, antioxidant, and antibacterial capabilities and finally the hepatotoxicity of the extract was evaluated. A total of ten phenolic compounds were identified via high-performance liquid chromatography coupled to diode array detector and mass spectrometer (HPLC–DAD–ESI/MSn): two phenolic acids (caffeic acid derivatives) and eight flavonoids (quercetin, kaempferol and isorhamnetin glycoside derivatives). Caftaric acid was the extract’s major phenolic constituent, accounting for more than 49% of the phenolic content, followed by quercetin-3-O-rutinoside (14.99%) and isorhamnetin-O-pentoside-O-rutinoside (9.56%). The total phenolic content found in the hydromethanolic extract was relevant (23.75 mg/g). A broad study of the extracts’ antioxidant activity was carried out, using a set of five distinct methods: two cell-based methods (1) oxidative haemolysis inhibition assay (OxHLIA); (2) the inhibition of the production of thiobarbituric acid reactive substances (TBARS); besides three chemical in vitro assays (3) reduction of the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH); (4) reduction of the 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonate) cation (ABTS) and (5) hydroxyl radical scavenging assay.2 Overall, the ora-pro-nobis leaf extract showed relevant values of antioxidant activity, even higher than Trolox in the DPPH and ABTS trials. The antioxidant activity verified for the hydroethanolic leaf extract of P. aculeata is possibly related to its phenolic composition; the predominant constituents of the extract, i.e. caftaric acid and rutin, have widely proven antioxidant activities.1 Furthermore, the evaluated extract showed no toxicity against a non-tumour liver primary culture PLP2, at the highest tested concentration (400 μg/mL). The antimicrobial activity exhibited by the extract against both Gram-positive (Enterococcus faecalis, Listeria monocytogenes, MRSA – Methicillin-resistant Staphylococcus aureus) and Gram-negative (Escherichia coli, Klebsiella pneumoniae, Morganella morganii, Proteus mirabilis, and Pseudomonas aeruginosa) bacteria (MIC values between 5 to 20 mg/mL) suggests the presence of a broad spectrum of phytochemicals with antimicrobial activity. The extract was more active against K. pneumonia than the antibiotic ampicillin, whereas against M. morganii it presented equivalent inhibitory effect. Therefore, the information reported here not only corroborates the importance of the production and consumption of P. aculeata leaves by the low income population to improve nutrition, but also reinforces their potential as a sustainable source of promising food ingredients to be used both for food enrichment and preservation.pt_PT
dc.description.sponsorshipThe authors are grateful to FCT - Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019), L. Barros, R. C. Calhelha and C. Pereira contracts (through national FCT funds, under the celebration of the contract program of institutional scientific employment). J. A. A. Garcia thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). R.C.G. Corrêa (Process number 167378/2017-1), R.M. Peralta (Project number 307944/2015-8) and A. Bracht (Project number 304090/2016-6) thank Conselho Nacional de Desenvolvimento Científico e Tecnologia (CNPq), Brazil, for the financial support. Funding: To FEDER-Interreg España-Portugal for financial support of the project 0377_Iberphenol_6_E and TRANSCoLAB 0612_TRANS_CO_LAB_2_P; FEDER under the Regional North Operational Program 2020, to the Mobilizing Project Norte-01-0247-FEDER-024479: ValorNatural®.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGarcia, Jessica A.A.; Corrêa, Rubia C.G.; Barros, Lillian; Pereira, Carla; Abreu, Rui M.V.; Alves, Maria José; Calhelha, Ricardo; Bracht, Adelar; Peralta, Rosane M.; Ferreira, Isabel C.F.R. (2019). Phytochemical profile and biological activities of 'Ora-pro-nobis' leaves (Pereskia aculeata Miller), an underexploited superfood from the Brazilian Atlantic Forest. In 11º Congresso Nacional de Cromatografia. Costa da Caparica. ISBN 978-989-8124-29-6pt_PT
dc.identifier.isbn978-989-8124-29-6
dc.identifier.urihttp://hdl.handle.net/10198/24051
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPereskia aculeata Millerpt_PT
dc.subjectBrazilian Atlantic Forestpt_PT
dc.titlePhytochemical profile and biological activities of 'Ora-pro-nobis' leaves (Pereskia aculeata Miller), an underexploited superfood from the Brazilian Atlantic Forestpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlaceCosta da Caparica, Portugalpt_PT
oaire.citation.title11º Encontro Nacional de Cromatografiapt_PT
oaire.fundingStream6817 - DCRRNI ID
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person.familyNameBarros
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person.familyNameCalhelha
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person.givenNameRúbia C.G.
person.givenNameLillian
person.givenNameCarla
person.givenNameRui M.V.
person.givenNameMaria José
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
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