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Phlomis fruticosa l. exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line

2022Journal article Open access
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dc.contributor.authorStojković, Dejan
dc.contributor.authorDrakulić, Danijela
dc.contributor.authorInês Dias, Maria
dc.contributor.authorZengin, Gokhan
dc.contributor.authorBarros, Lillian
dc.contributor.authorIvanov, Marija
dc.contributor.authorGašić, Uroš
dc.contributor.authorRajčević, Nemanja
dc.contributor.authorStevanović, Milena
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorSoković, Marina
dc.date.accessioned2022-03-02T16:30:41Z
dc.date.available2022-03-02T16:30:41Z
dc.date.issued2022
dc.description.abstractDespite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa ex-tract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic ef-fect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.pt_PT
dc.description.sponsorshipThis work has been supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (451-03-9/2021-14/200007 and 451-03-68/2022-14/200042). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the institutional scien-tific employment program-contract for M.I. Dias and L. Barros; to FEDER-Interreg España-Portugal program for financial support through the project 0377_Iberphenol_6_E and TRANSCoLAB 0612_TRANS_CO_LAB_2_P.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationStojković, Dejan; Drakulić, Danijela; Inês Dias, Maria; Zengin, Gokhan; Barros, Lillian; Ivanov, Marija; Gašić, Uroš; Rajčević, Nemanja; Stevanović, Milena; Ferreira, Isabel C.F.R.; Soković, Marina (2022). Phlomis fruticosa l. exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell line. EXCLI Journal. ISSN 1611-2156. 21, p. 387-399pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/25133
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPhlomis fruticosapt_PT
dc.subjectAntioxidantpt_PT
dc.subjectAntineurodegenerativept_PT
dc.subjectGlioblastomapt_PT
dc.subjectProoxidantpt_PT
dc.subjectPhenolic compoundspt_PT
dc.titlePhlomis fruticosa l. exerts in vitro antineurodegenerative and antioxidant activities and induces prooxidant effect in glioblastoma cell linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.endPage399pt_PT
oaire.citation.startPage387pt_PT
oaire.citation.titleEXCLI Journalpt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias
person.familyNameBarros
person.familyNameFerreira
person.givenNameMaria Inês
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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