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Viola cornuta and Viola x wittrockiana: phenolic compounds, antioxidant and neuroprotective activities on Caenorhabditis elegans

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dc.contributor.authorMoliner, Cristina
dc.contributor.authorBarros, Lillian
dc.contributor.authorDias, Maria Inês
dc.contributor.authorReigada, Inés
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorLópez, Víctor
dc.contributor.authorLanga, Elisa
dc.contributor.authorRincón, Carlota Gómez
dc.date.accessioned2018-02-19T10:00:00Z
dc.date.accessioned2020-01-06T14:09:42Z
dc.date.available2018-01-19T10:00:00Z
dc.date.available2020-01-06T14:09:42Z
dc.date.issued2019
dc.description.abstractDifferent Viola species are known for their traditional use as analgesic, antitussive, febrifuge, hipnotic, analgesic and anti-inflammatory medicinal agents. Additionally, they are considered edible flowers in certain cultures. Thus, the aim of this work was to characterize the phenolic composition and to assess the neuroprotective properties of Viola cornuta and Viola x wittrockiana using in vitro and in vivo methodologies with Caenorhabditis elegans as model. The identification of the phenolic compounds was carried out with a LCDAD- ESI/MSn. The antioxidant activity of the extracts was determined in vitro using Folin- Ciocalteu, DPPH and FRAP assays and in vivo with a juglone-induced oxidative stress in C. elegans. The neuroprotective properties were evaluated measuring the ability to inhibit CNS enzymes (MAO A, AChE), and the capability to avoid paralyzing the C. elegans CL4176, an Alzheimer disease model. The phenolic content was higher in V. x wittrockiana, being quercetin-3-O-(6-O-rhamnosylglucoside)-7-O-rhamnoside the predominant compound in the extract, which also exhibited a stronger antioxidant capacity in vitro and a higher response to lethal oxidative stress on C. elegans than V. cornuta. Only V. x wittrockiana showed inhibitory effect on CNS enzymes, such as acetylcholinesterase and monoamine oxidase A, but both had protective effect against the paralysis of C. elegans. These findings suggest that the studied V. cornuta and V. x wittrockiana could be interesting candidates for age related neurodegenerative disorder associated with oxidative stress.
dc.description.sponsorshipUniversidad San Jorge is acknowledged for financial support and providing Cristina Moliner with a PhD scholarship. Innoflower S.L. is thanked for supplying fresh flowers. The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and L. Barros contract; to FEDER-Interreg Espa~na-Portugal programme for financial support through the project 0377_Iberphenol_6_E.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationMoliner, Cristina; Barros, Lillian; Dias, Maria Inês; Reigada, Inés; Ferreira, Isabel C.F.R.; López, Víctor; Langa, Elisa; Rincón, Carlota Gómez (2019). Viola cornuta and Viola x wittrockiana: Phenolic compounds, antioxidant and neuroprotective activities on Caenorhabditis elegans. Journal of Food and Drug Analysis. ISSN 1021-9498. 27, p. 849-859en_EN
dc.identifier.doi10.1016/j.jfda.2019.05.005en_EN
dc.identifier.issn1021-9498
dc.identifier.urihttp://hdl.handle.net/10198/20244
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.subjectAntioxidanten_EN
dc.subjectCaenorhabditis elegansen_EN
dc.subjectLC-DAD-ESI/MSnen_EN
dc.subjectNeuroprotective potentialen_EN
dc.subjectPolyphenolsen_EN
dc.subjectViolaen_EN
dc.titleViola cornuta and Viola x wittrockiana: phenolic compounds, antioxidant and neuroprotective activities on Caenorhabditis elegansen_EN
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUID/AGR/00690/2013
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.fundingStream5876
person.familyNameBarros
person.familyNameDias
person.familyNameFerreira
person.givenNameLillian
person.givenNameMaria Inês
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridM-8242-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typearticleen_EN
relation.isAuthorOfPublication3af07ffe-f914-48ba-a5d5-efcf70fdce01
relation.isAuthorOfPublication7ca8432b-e7f4-4116-a56e-9eba0d4403bd
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
relation.isAuthorOfPublication.latestForDiscovery7ca8432b-e7f4-4116-a56e-9eba0d4403bd
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