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A novel molecular diagnostic method for the gut content analysis of Philaenus DNA

2022Artigo científico Acesso aberto
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dc.contributor.authorRodrigues, Isabel
dc.contributor.authorRamos, Vitor
dc.contributor.authorBenhadi-Marín, Jacinto
dc.contributor.authorMoreno, Aránzazu
dc.contributor.authorFereres, Alberto
dc.contributor.authorPereira, J.A.
dc.contributor.authorBaptista, Paula
dc.date.accessioned2022-03-17T12:11:58Z
dc.date.available2022-03-17T12:11:58Z
dc.date.issued2022
dc.description.abstractPhilaenus spumarius is a vector of Xylella fastidiosa, one of the most dangerous plants pathogenic bacteria worldwide. There is currently no control measure against this pathogen. Thus, the development of vector control strategies, like generalist predators, such as spiders, could be essential to limit the spread of this vector-borne pathogen. In this study, a polymerase chain reaction (PCR)-based approach was developed to principally detect DNA of P. spumarius in the spider’s gut. Accordingly, 20 primer pairs, targeting the mitochondrial cytochrome oxidase I (COI) and cytochrome b (cytB) genes, were tested for specificity, sensitivity, and efficiency in detecting P. spumarius DNA. Overall, two primer sets, targeting COI gene (COI_Ph71F/COI_Ph941R) and the cytB gene (cytB_ Ph85F/cytB_Ph635R), showed the highest specificity and sensitivity, being able to amplify 870 pb and 550 bp fragments, respectively, with P. spumarius DNA concentrations 100-fold lower than that of the DNA of non-target species. Among these two primer sets, the cytB_Ph85F/cytB_Ph635R was able to detect P. spumarius in the spider Xysticus acerbus, reaching 50% detection success 82 h after feeding. The feasibility of this primer set to detect predation of P. spumarius by spiders was confirmed in the field, where 20% of the collected spiders presented positive amplifications.pt_PT
dc.description.sponsorshipThis work was supported by the EU H2020 Research Project XF-ACTORS “Xylella fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy” (Grant Agreement 727987) and to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020). Isabel Rodrigues also acknowledges the PhD research Grant (2020.07051.BD) provided by FCT.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRodrigues, Isabel; Ramos, Vítor; Benhadi Marín, Jacinto; Moreno, Aránzazu; Fereres, Alberto; Pereira, J.A.; Baptista, Paula (2022). A novel molecular diagnostic method for the gut content analysis of Philaenus DNA. Scientific Reports. ISSN 2045 2322. 12:1, p. 1-9pt_PT
dc.identifier.doi10.1038/s41598-021-04422-1pt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10198/25244
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNaturept_PT
dc.relationXylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectDNApt_PT
dc.subjectDNA primerspt_PT
dc.subjectFeeding behaviopt_PT
dc.titleA novel molecular diagnostic method for the gut content analysis of Philaenus DNApt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber727987
oaire.awardNumberUIDB/00690/2020
oaire.awardTitleXylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/727987/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRodrigues
person.familyNameRamos
person.familyNameBenhadi-Marín
person.familyNamePereira
person.familyNameBaptista
person.givenNameIsabel
person.givenNameVitor
person.givenNameJacinto
person.givenNameJosé Alberto
person.givenNamePaula
person.identifier.ciencia-idB115-33B3-B059
person.identifier.ciencia-id8D12-5415-20E0
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.ciencia-id7D11-FE1E-CD0F
person.identifier.orcid0000-0002-4827-2115
person.identifier.orcid0000-0002-5824-3633
person.identifier.orcid0000-0002-9804-4145
person.identifier.orcid0000-0002-2260-0600
person.identifier.orcid0000-0001-6331-3731
person.identifier.ridA-4540-2011
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id35389568900
person.identifier.scopus-author-id57204366348
person.identifier.scopus-author-id14051688000
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication90201f77-795e-4756-a469-c94f7ec47929
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