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Phenolic composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) seeds

2019Journal article Open access
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dc.contributor.authorAlonso-Esteban, José Ignacio
dc.contributor.authorPinela, José
dc.contributor.authorBarros, Lillian
dc.contributor.authorĆirić, Ana
dc.contributor.authorSoković, Marina
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorTorija-Isasa, Esperanza
dc.contributor.authorCortes Sánchez-Mata, María de
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-05-02T10:00:29Z
dc.date.available2019-05-02T10:00:29Z
dc.date.issued2019
dc.description.abstractHop (Humulus lupulus L.) is a perennial climbing plant cultivated for commercial use, mainly in brewing industry, since the female inflorescences (cones) contain lupulin glands that confer the characteristic bitterness, aroma and flavour to beer. However, the bioactive potential of the seeds found in these cones remains unexplored. In this study, the phenolic composition of hop seeds was analysed by LC-DAD-ESI/MS n and its antioxidant, cytotoxic and antimicrobial activities were screened against oxidizable substrates, human tumour and non-tumour cell lines, and different bacteria and fungi, respectively. (+)-Catechin and (-)-epicatechin were identified in the seeds, totalizing 17.6 ± 0.7 mg/g extract. The seed extract showed antioxidant activity mainly through the formation inhibition of thiobarbituric acid reactive substances (TBARS), and had cytotoxic effects against MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer), HeLa (cervical carcinoma), and HepG2 (hepatocellular carcinoma) tumour cells, but no toxicity towards the normal PLP2 cell line was observed. The extract showed remarkable antimicrobial properties, being better than the positive controls against almost all tested bacteria and fungi of the genus Penicillium. These results highlight the potential of hop seeds as a source of functional ingredients with applications in the food and pharmaceutical sectors.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO ( UID/AGR/00690/2019 ) and the research contracts of J. Pinela (Project AllNatt, POCI-01-0145-FEDER-030463), L. Barros, and R.C. Calhelha. Also to ALIMNOVA-UCM research group and Art. 83 project ref: UCM 252/2017, and to the Rafael Folch Foundation for the J.I. Alonso-Esteban grant (2016/01M). This work was funded by the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project NORTE-01-0145-FEDER-023289: DeCodE. To FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E. Authors are grateful also to the Ministry of Education, Science and Technological Development of Serbia (Grant No. OI 173,032).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlonso-Esteban, José Ignacio; Pinela, José; Barros, Lillian; Ćirić, Ana; Soković, Marina; Calhelha, Ricardo C.; Torija-Isasa, Esperanza; Cortes Sánchez-Mata, María de; Ferreira, Isabel C.F.R. (2019). Phenolic composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) seeds. Industrial Crops and Products. ISSN 0926-6690. 134, p. 154-159pt_PT
dc.identifier.doi10.1016/j.indcrop.2019.04.001pt_PT
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10198/19288
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectCatechin/epicatechinpt_PT
dc.subjectCytotoxic activitypt_PT
dc.subjectHop seedspt_PT
dc.titlePhenolic composition and antioxidant, antimicrobial and cytotoxic properties of hop (Humulus lupulus L.) seedspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.endPage159pt_PT
oaire.citation.startPage154pt_PT
oaire.citation.titleIndustrial Crops and Productspt_PT
oaire.citation.volume134pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinela
person.familyNameBarros
person.familyNameCalhelha
person.familyNameFerreira
person.givenNameJosé
person.givenNameLillian
person.givenNameRicardo C.
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-idF313-E3CE-554E
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0002-6801-4578
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridB-4466-2014
person.identifier.ridJ-3600-2013
person.identifier.ridJ-2172-2014
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id6507978333
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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