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Exploring the therapeutic potential of Quercus ilex acorn extract in papillomavirus-induced lesions

2024Journal article Open access
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dc.contributor.authorMedeiros-Fonseca, Beatriz
dc.contributor.authorFaustino-Rocha, Ana
dc.contributor.authorPires, Maria João
dc.contributor.authorNeuparth, Maria João
dc.contributor.authorVala, Helena
dc.contributor.authorVasconcelos-Nóbrega, Cármen
dc.contributor.authorGouvinhas, Irene
dc.contributor.authorBarros, Ana Novo
dc.contributor.authorDias, Maria Inês
dc.contributor.authorBarros, Lillian
dc.contributor.authorBastos, Margarida M.S.M.
dc.contributor.authorGonçalves, Lio
dc.contributor.authorFélix, Luís
dc.contributor.authorVenâncio, Carlos
dc.contributor.authorMedeiros, Rui
dc.contributor.authorCosta, Rui Miguel Gil
dc.contributor.authorOliveira, Paula A.
dc.date.accessioned2025-01-13T10:41:05Z
dc.date.available2025-01-13T10:41:05Z
dc.date.issued2024
dc.description.abstractPapillomaviruses (PVs) infections have been documented in numerous animal species across different regions worldwide. They often exert significant impacts on animal health and livestock production. Scientists have studied natural products for over half a century due to their diverse chemical composition, acknowledging their value in fighting cancer. Acorns (Quercus ilex) are believed to have several unexplored pharmacological properties. This study aimed to evaluate the in vivo safety and cancer chemopreventive activity of an infusion extract of Q. ilex in a transgenic mouse model of human PV (HPV)-16, which developed squamous cell carcinomas through a multistep process driven by HPV16 oncogenes. Q. ilex extract was prepared by heating in water at 90°C and then characterized by mass spectrometry. Phenolic compounds from this extract were administered in drinking water to female mice in three different concentrations (0.03, 0.06, and 0.09 g/mL) over a period of 28 consecutive days. Six groups (n = 6) were formed for this study: group 1 (G1, wildtype [WT], water), group 2 (G2, HPV, water), group 3 (G3, WT, 0.09 g/mL), group 4 (G4, HPV, 0.03 g/mL), group 5 (G5, HPV, 0.06 g/ mL), and group 6 (G6, HPV, 0.09 g/mL). Throughout the experiment, humane endpoints, body weight, food intake, and water consumption were recorded weekly. Following the experimental period, all mice were sacrificed, and blood, internal organs, and skin samples were collected. Blood was used to measure glucose and microhematocrit and later biochemical parameters, such as creatinine, urea, albumin, alanine aminotransferase, and total proteins. Histological analysis was performed on skin and organ samples. The administration of Q. ilex extract resulted in a statistically significant increase in relative organ weight among HPV transgenic animals, indicating adaptive biological response to the tested concentrations. Moreover, a reduction in characteristic skin lesions was observed in animals treated with the 0.06 and 0.09 g/mL extract. These results provide a favorable chemopreventive profile for Q. ilex extract at concentrations of 0.06 and 0.09 g/mL. This study highlights the potential of Q. ilex extract as a safe and effective therapeutic strategy against HPV16- associated lesions in transgenic mouse models. The limitation of our study was the durability of transgenic animals. As a more sensitive species, we must always be careful with the durability of the test. We intend to study concentrations of 0.06 and 0.09 g/mL for longer to further investigate their possible effects.pt_PT
dc.description.sponsorshipBeatriz Medeiros-Fonseca received a Ph.D. grant number [2020.07675.BD] (https://doi. org/10.54499/2020.07675.BD) financed by FCT (Foundation for Science and Technology) through FSE (European Social Fund). This work was supported by National Funds by FCT-Portuguese under the following projects: CITAB [UID/04033/2020] (https:// doi.org/10.54499/UIDB/04033/2020); CERNAS [UIDB/00681/2020] (https://doi.org/10.54499/ UIDB/00681/2020); LEPABE [UIDB/00511/2020] (https://doi.org/10.54499/UIDB/00511/2020) and [UIDP/00511/2020] (https://doi.org/10.54499/ UIDP/00511/2020); ALiCE [LA/P/0045/2020] (https://doi.org/10.54499/LA/P/0045/2020); CIMO [UIDB/00690/2020] (https://doi.org/10.54499/ UIDB/00690/2020) and [UIDP/00690/2020] (https:// doi.org/10.54499/UIDP/00690/2020); SusTEC, [LA/P/0007/2020] (https://doi.org/10.54499/ LA/P/0007/2020); by national funds through the FCT/ MCTES (PIDDAC). Furthermore, national funding by FCT/P.I., through the institutional scientific employment program contract for Lillian Barros (https:// doi.org/10.54499/CEECINST/00107/2021/CP2793/ CT0002), Maria I. Dias (https://doi.org/10.54499/ CEECINST/00016/2018/CP1505/CT0004), Luís Félix (https://doi.org/10.54499/2021.00458.CEECIND/ CP1690/CT0001), Irene Gouvinhas (https://doi. org/10.54499/2022.00498.CEECIND/CP1749/ CT0001) contracts.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMedeiros-Fonseca, Beatriz; Faustino-Rocha, Ana I.; Pires, Maria João; Neuparth, Maria João; Vala, Helena; Vasconcelos-Nóbrega, Cármen; Gouvinhas, Irene; Barros, Ana Novo; Dias, Maria Inês; Barros, Lillian; Bastos, Margarida M. S. M.; Gonçalves, Lio; Félix, Luís; Venâncio, Carlos; Medeiros, Rui; Costa, Rui Miguel Gil da; Oliveira, Paula A. (2024). Exploring the therapeutic potential of Quercus ilex acorn extract in papillomavirus-induced lesions. Veterinary World. ISSN 2231-0916. 17:11, p. 2644-2658pt_PT
dc.identifier.doi10.14202/vetworld.2024.2644-2658pt_PT
dc.identifier.eissn0972-8988
dc.identifier.issn2231-0916
dc.identifier.urihttp://hdl.handle.net/10198/30927
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherVeterinary Worldpt_PT
dc.relationEstudo dos efeitos imunossupressores de Pteridium aquilinum e suas contribuições para o processo de carcinogénese induzido por papilomavírus.
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
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dc.relationMenthol and Thymol loaded in mucoadhesive nanocapsules for the transportation of ornamental fish
dc.relationA sustainable and innovative strategy in wound management: development of a healing bio-based formulation resorting to winery by-products bioactive compounds
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAcornpt_PT
dc.subjectAntioxidant capacitypt_PT
dc.subjectCancerpt_PT
dc.subjectMouse modelpt_PT
dc.subjectPolyphenolspt_PT
dc.subjectQuercus spppt_PT
dc.titleExploring the therapeutic potential of Quercus ilex acorn extract in papillomavirus-induced lesionspt_PT
dc.typejournal article
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oaire.citation.endPage2658pt_PT
oaire.citation.issue11pt_PT
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oaire.citation.titleVeterinary Worldpt_PT
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