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Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts

2012Journal article Open access
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dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorBarros, Lillian
dc.contributor.authorMartins, Anabela
dc.contributor.authorQueiroz, Maria João R.P.
dc.contributor.authorSantos-Buelga, Celestino
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2013-01-04T14:48:33Z
dc.date.available2013-01-04T14:48:33Z
dc.date.issued2012
dc.description.abstractGanoderma lucidum is one of the most extensively studied mushrooms due to its medicinal properties. Herein, a systematic study was carried out in order to compare the antioxidant activity of phenolic and polysaccharidic extracts from fruiting body, spores and mycelium, obtained in three different culture media, of G. lucidum from Northeast Portugal. Phenolic extracts were characterized using high-performance liquid chromatography coupled to photodiode array detection, while polysaccharidic extracts were hydrolysed and further characterized using HPLC and refraction index detection. In general, the phenolic extracts (Ph) proved to have higher antioxidant potential than their corresponding polysaccharidic extracts (Ps). Amongst phenolic extracts, FB-Ph provided the highest antioxidant activity (EC50 ≤ 0.6 mg/ml) and the highest content in total phenolics (~29 mg GAE/g extract) and phenolic acids (p-hydroxybenzoic and p-coumaric acids). S-Ps was the polysaccharidic extract with the best antioxidant activity (EC50 ≤ 2 mg/ml); nevertheless, the highest levels of total phenolics were obtained in FB-PS (~56 mg GAE/g extract), while the highest levels of total polysaccharides (~14 mg PE/g extract) and individual sugars were observed in mycelia obtained from solid culture media, M-PDA-Ps and M-sMMN-Ps. The free radical scavenging properties, reducing power and lipid peroxidation inhibition of G. lucidum seemed to be correlated with phenolic compounds mostly in a free form, but also linked to polysaccharides.por
dc.identifier.citationHeleno, Sandrina A.; Barros, Lillian; Martins, Anabela; Queiroz, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R. (2012). Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: a comparative study of the antioxidant potential of phenolic and polysaccharidic extracts. Food Research International. ISSN 0963-9969. 46:1, p. 135-140por
dc.identifier.doi10.1016/j.foodres.2011.12.009
dc.identifier.issn0963-9969
dc.identifier.urihttp://hdl.handle.net/10198/7816
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBD/70304/2010
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.subjectGanoderma lucidumpor
dc.subjectFruiting body/spores/myceliumpor
dc.subjectPhenolic/polysaccharidic extractspor
dc.subjectAntioxidant propertiespor
dc.titleFruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extractspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.citation.endPage140por
oaire.citation.startPage135por
oaire.citation.titleFood Research Internationalpor
oaire.citation.volume46por
oaire.fundingStream5876-PPCDTI
person.familyNameHeleno
person.familyNameBarros
person.familyNameMartins
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameLillian
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8B18-3095-6FC9
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridJ-3600-2013
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicatione89b5bf5-d315-49b6-871e-fa8eb6030676
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