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Phenolic Class Analysis in Honey: Comparison of Classical and Single UV Spectrum Methodologies

2024Journal article Open access
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dc.contributor.authorPaula, Vanessa B.
dc.contributor.authorSousa-Dias, Miguel L.
dc.contributor.authorSeixas, Natália
dc.contributor.authorCombarros-Fuertes, Patricia
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorDias, L.G.
dc.date.accessioned2024-12-20T16:33:52Z
dc.date.available2024-12-20T16:33:52Z
dc.date.issued2024
dc.description.abstractThe analytical results from a study of 16 honey samples (extra white to dark honey color range) of phenolic compounds obtained using the single UV spectrum methodology and classical spectrophotometric methods (Folin–Ciocalteu and AlCl3 methods) are presented. The first method quantified all classes of phenolic compounds in honey’s SPE-C18 extract: the total hydroxybenzoic acid content (concentrations between 0.37 ± 0.05 and 4.46 ± 0.37 mg of gallic acid/g of honey), total hydroxycinnamic acid content (0.13 ± 0.03 and 2.76 ± 0.13 mg of ferulic acid/g of honey), and total flavonoid content (0.15 ± 0.03 and 1.63 ± 0.17 mg of quercetin/g of honey). The total phenolic contents were, on average, 1.86 ± 0.72 and 1.78 ± 0.79 times higher than the results obtained for raw honey and the SPE-C18 extract, respectively, using the classical Folin–Ciocalteu method. The total flavonoid contents, on average, were 6.02 ± 3.14 times larger and 0.66 ± 0.33 times smaller than the results obtained using the classical AlCl3 method for raw honey and SPE-C18 extract, respectively.pt_PT
dc.description.sponsorshipThis work was supported by national funds through FCT/MCTES (PIDDAC): CIMO, UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020), and UIDP/00690/2020 (DOI: 10.54499/ UIDP/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPaula, Vanessa B.; Sousa-Dias, Miguel L.; Seixas, Natália L.; Combarros-Fuertes, Patricia; Estevinho, Letícia M.; Dias, Luís G. (2024). Phenolic Class Analysis in Honey: Comparison of Classical and Single UV Spectrum Methodologies. Processes. ISSN 2227-9717. 12:10, p. 1-17pt_PT
dc.identifier.doi10.3390/pr12102297pt_PT
dc.identifier.issn2227-9717
dc.identifier.urihttp://hdl.handle.net/10198/30776
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectRaw honeypt_PT
dc.subjectHoney SPE-C18 extractspt_PT
dc.subjectPhenolic compounds groupspt_PT
dc.subjectSingle UV spectrum methodologypt_PT
dc.subjectFolin–Ciocalteu methodpt_PT
dc.subjectAlCl3 methodpt_PT
dc.titlePhenolic Class Analysis in Honey: Comparison of Classical and Single UV Spectrum Methodologiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage17pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleProcessespt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.affiliation.nameInstituto Politécnico de Bragança
person.familyNamePaula
person.familyNameSeixas
person.familyNameEstevinho
person.familyNameDias
person.givenNameVanessa B.
person.givenNameNatália
person.givenNameLetícia M.
person.givenNameLuís G.
person.identifier.ciencia-id5F16-A866-562D
person.identifier.ciencia-idC816-E8C4-7D3F
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.orcid0000-0001-7281-8598
person.identifier.orcid0000-0002-9249-1948
person.identifier.orcid0000-0002-1210-4259
person.identifier.scopus-author-id6506577664
person.identifier.scopus-author-id23569169900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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