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Optimisation of stability and colouring power of double emulsion systems loaded with Daucus carota L.

2024Conference object Open access
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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.sdg03:Saúde de Qualidade
datacite.subject.sdg02:Erradicar a Fome
dc.contributor.authorTeixeira, Liandra Gracher
dc.contributor.authorSilva, Samara Cristina
dc.contributor.authorColucci, Giovana
dc.contributor.authorSantamaria-Echart, Arantzazu
dc.contributor.authorPeres, António M.
dc.contributor.authorDias, Madalena M.
dc.contributor.authorBarreiro, Filomena
dc.date.accessioned2025-11-05T17:01:23Z
dc.date.available2025-11-05T17:01:23Z
dc.date.issued2024
dc.description.abstractAnthocyanins (ACNs) are water-soluble bioactive flavonoids found in flowers, roots, leaves, seeds, and stalks. ACNs cover a wide palette of colours, from blue and purple through orange to red1,2. They are used in the food industry (food colourant with E163 code), namely in fruit juice concentrates, nectars, jellies, yoghurts, marmalades, potato chips, ice creams, and soft drinks, replacing the synthetic Red 40 colourants. They also have diversified pharmaceutical and cosmetic uses due to their colouring and antioxidant attributes. However, their instability due to pH, light, and temperature is a major concern. This study used a double water-in-oil-in-water (W1/O/W2) emulsion technique to prevent rapid colour loss and colour variability with pH of a commercial black carrot extract (Fig. 1). A Central Composite Rotatable Design (CCRD) 22 was used to examine the effects of colourant concentration (wt%) and emulsion ratio ((W1/O)/W2) on stability parameters (droplet size (D4,3), creaming index (CI), and colourimetric values (L*, a*). The main objective was to find an optimised formulation in terms of colourant potential and emulsion stability. Measurements were taken at 1, 7, 15, and 30 days. The optimal experimental design revealed two optimal solutions: 1) 48/52 (W1/O)/W2 ratio with 6 wt.% colorant, and 2) 41/59 (W1/O)/W2 ratio with 11 wt.% colourant. These formulations showed lower creaming index and droplet sizes with higher colourant potential. After accounting for the effect of time, the optimal condition was 6 wt% colourant concentration and 48/52 v/v (W1/O)/W2 emulsion ratio. A stable colour was maintained after 30 days (L*: 44.11±0.03, a*: 25.79±0.01, D4;3: 9.62 ± 0.1 µm, and CI: 14.55±0.99%). These findings are promising and might be applicable to produce novel colourants to be applied in several industries, such as food and cosmetics.eng
dc.description.sponsorshipWe thank the financial support through national funds FCT/MCTES (PIDDAC) to CIMO UIDB/00690/2020 (DOI:10.54499/UIDB/00690/2020) and UIDP/00690/2020 (DOI:10.54499/UIDP/00690/2020); SusTEC LA/P/0007/2020 (DOI:10.54499/LA/P/0007/2020); LSRE-LCM UIDB/50020/2020 (DOI:10.54499/UIDB/50020/2020) and UIDP/50020/2020 (DOI:10.54499/UIDP/50020/2020); and ALiCE LA/P/0045/2020 (DOI:10.54499/LA/P/0045/2020). National funding by FCT, P.I., through the institutional scientific employment program contract of A. Santamaria-Echart. FCT for the PhD research grant of Liandra Gracher-Teixeira 2020.08803.BD (DOI: 10.54499/2020.08803.BD), Samara C. Silva (SFRH/BD/148281/2019), and Giovana Colucci (2021.05215.BD)
dc.identifier.citationTeixeira, Liandra Gracher; Silva, Samara Cristina; Colucci, Giovana; Santamaria-Echart, Arantzazu; Peres, António M.; Dias, Madalena M.; Barreiro, M.F. (2024). Optimisation of stability and colouring power of double emulsion systems loaded with Daucus carota L.. In 10th Iberian Meeting on Colloids and Interfaces: Book of Abstracts. Coimbra
dc.identifier.urihttp://hdl.handle.net/10198/34965
dc.language.isoeng
dc.peerreviewedyes
dc.publisherRICI
dc.relationMountain Research Center
dc.relationLaboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials
dc.relationALICE - Associate Laboratory in Chemical Engineering
dc.relationCo-encapsulation of natural colorants for color and bioactivity modulation
dc.relationMountain Research Center
dc.relationLaboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials
dc.relationDevelopment of an integrated approach to produce phycocyanin and valorize co-bioproducts from Spirulina Arthrospira platensis
dc.relationLignin-based Pickering stabilisers for innovative cosmeceutical formulations
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDaucus carota L.
dc.titleOptimisation of stability and colouring power of double emulsion systems loaded with Daucus carota L.por
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleLaboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials
oaire.awardTitleALICE - Associate Laboratory in Chemical Engineering
oaire.awardTitleCo-encapsulation of natural colorants for color and bioactivity modulation
oaire.awardTitleMountain Research Center
oaire.awardTitleLaboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials
oaire.awardTitleDevelopment of an integrated approach to produce phycocyanin and valorize co-bioproducts from Spirulina Arthrospira platensis
oaire.awardTitleLignin-based Pickering stabilisers for innovative cosmeceutical formulations
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50020%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0045%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.08803.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50020%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F148281%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.05215.BD/PT
oaire.citation.conferenceDate2024
oaire.citation.conferencePlaceCoimbra
oaire.citation.endPage211
oaire.citation.startPage211
oaire.citation.title10th Iberian Meeting on Colloids and Interfaces: Book of Abstracts
oaire.fundingStream6817 - DCRRNI ID
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oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameTeixeira
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person.familyNamePeres
person.familyNameBarreiro
person.givenNameLiandra Gracher
person.givenNameGiovana
person.givenNameArantzazu
person.givenNameAntónio M.
person.givenNameFilomena
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