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Enhanced extraction of ergosterol from Pleurotus ostreatus using response surface methodology (RSM)

2019Conference object Open access
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dc.contributor.authorTaofiq, Oludemi
dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorCarocho, Márcio
dc.contributor.authorCosta, Cristina
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Joana
dc.contributor.authorFerreira, Inês
dc.contributor.authorNunes, João
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-06-19T14:41:22Z
dc.date.available2019-06-19T14:41:22Z
dc.date.issued2019
dc.description.abstractPleurotus ostreatus (Jacq. ex Fr.) P. Kumm., is one of the most widely consumed mushrooms in the world with interesting health-promoting benefits, mainly due to its richness in several bioactive compounds [1]. Mushrooms produce ergosterol as one of their main sterols, which has been considered a contributor to their anti-inflammatory, antioxidant, and antitumor properties [2]. Obtaining an ergosterol enriched extract depends on different variables, such as the extraction method, solvent type, temperature, extraction time, and the solid-liquid ratio [3]. Therefore, it is essential to define the main variables and relevant response criteria to maximize the extraction yield and purity, combining the economic competitivity. In the present work, response surface methodology (RSM) was applied to optimize a heat assisted extraction system (HAE), combining time (t) and temperature (T) effects, and using a circumscribed central composite design (CCCD) for the recovery of ergosterol from the fruiting bodies of P. ostreatus produced with lignocellulose substract. The obtained responses were the quantification of ergosterol by HPLC-UV (Y1: mg of ergosterol per g of extract residue and Y2: mg of ergosterol per 100 g of dry weight mushroom), and the extraction yield (Y3: %). The CCCD consist of 16 response combinations and 4 centre points. Response surface models were fitted by using the following second order polynomial equation. The results obtained showed a significant interaction between the variables. For all the three responses (Y1, Y2, and Y3), the model successfully explained more than 80% variation in the experimental data (i.e. R2˃8). The individual optimum conditions and responses were as follows; Y1 (10 min, 30°C, 57.6 mg/g), Y2 (150 min, 61°C, 246.3 mg/100 g dw), and Y3 (10 min, 80.9°C, 9.3%). The global optimum conditions predicted by the model were: 150 min and 54.3 °C, capable of yielding 7.3 %, 33.3 mg/g and 244.3 mg/100 g dw. The values predicted by the model are in close agreement with the experimental observations with very low residual distribution, proving the validity of the applied model. The results also showed the usefulness of the predictions for future scale up based on the desired responses. The obtained ergosterol enriched extract can be considered as a bioactive ingredient for pharmaceutical, cosmeceutical and nutraceutical purposes.pt_PT
dc.description.sponsorshipFCT for financial support to CIMO (UID/AGR/00690/2013), M. Carocho (SFRH/BPD/114650/2016) and S. Heleno’s (SFRH/BPD/101413/2014) grants, and L. Barros’ contract; European Agricultural Fund for Rural Development (EAFRD), through the Rural Development Program (PDR2020), within the scope of Project MicoCoating (PDR2020-101-031472); Xunta de Galicia for financial support to M.A. Prieto. This work was also funded by the European Structural and Investment Funds (FEEI) through the Regional Operational Program North 2020, within the scope of ProjectMobilizadorValorNatural®pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTaofiq, Oludemi; Heleno, Sandrina; Carocho, Márcio; Costa, Cristina; Prieto, M.A.; Barros, Joana; Ferreira, Inês; Nunes, João; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Enhanced extraction of ergosterol from Pleurotus ostreatus using response surface methodology (RSM). In XX EuroFoodChem Conference. Porto. ISBN 978-989-8124-26-5pt_PT
dc.identifier.isbn978-989-8124-26-5
dc.identifier.urihttp://hdl.handle.net/10198/19349
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationNatural food preservatives from plant sources: extraction, stabilization and applications development
dc.relationObtaining of mycosterols to develop new functionalized foods: extraction, refinement, stabilization and incorporation into dairy products
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleEnhanced extraction of ergosterol from Pleurotus ostreatus using response surface methodology (RSM)pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleNatural food preservatives from plant sources: extraction, stabilization and applications development
oaire.awardTitleObtaining of mycosterols to develop new functionalized foods: extraction, refinement, stabilization and incorporation into dairy products
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F114650%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F101413%2F2014/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleXX EuroFoodChem Conferencept_PT
oaire.fundingStream5876
oaire.fundingStreamFARH
person.familyNameHeleno
person.familyNameCarocho
person.familyNamePrieto
person.familyNameBarros
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameMárcio
person.givenNameMiguel A.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierawbWiiMAAAAJ&hl
person.identifier107688
person.identifier469085
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person.identifier.ciencia-id8B18-3095-6FC9
person.identifier.ciencia-id5614-005B-5CF1
person.identifier.ciencia-id221E-1E00-AB74
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-8978-4547
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id52463229800
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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