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Advisor(s)
Abstract(s)
A técnica de inseminação artificial (IA) está mais difundida entre os caprinos do que entre os ovinos. Nos caprinos, os resultados da aplicação desta técnica promovem, normalmente, melhores taxas reprodutivas. Nestes animais, é mais fácil entrar e até ultrapassar o canal cervical, pelo que a IA tende a ser mais profunda. Consequentemente, o número de espermatozóides viáveis que devem estar em cada dose seminal é menor, possibilitando a preparação de um maior número de doses seminais por ejaculado. Depois de recolhido e avaliado, o sémen pode ser usado fresco, refrigerado ou congelado. O processo de preservação do sémen condiciona fortemente os resultados reprodutivos.
O presente estudo teve com principal objectivo comparar a eficácia da aplicação da técnica de IA com sémen fresco (37ºC) ou refrigerado (15ºC) em cabras da raça Serrana. Vinte e oito cabras adultas foram inseminadas com sémen fresco e 29 com sémen refrigerado. A actividade ovárica foi sincronizada através de um tratamento progestagénico curto e a ovulação foi estimulada administrando eCG. O sémen foi recolhido por electroejaculação. Depois de avaliado, o sémen foi diluído com diluidores comerciais. O sémen fresco foi mantido a 37ºC até ao momento da IA. O sémen refrigerado foi arrefecido num banho-maria refrigerado (2 horas). Neste caso, o enchimento das palhinhas francesas foi feito a 15ºC. Cada dose seminal continha, pelo menos, 80 x 106 espermatozóides/ ml. A IA foi feita a tempo fixo – 43 ± 1 hora pós-administração de eCG. A IA foi o mais profunda possível. Os resultados estão ainda em análise.
Artificial insemination (AI) is more common among goats than ewes. Better reproductive rates are to be expected between goats. Cervix anatomy is more suitable to AI in goats than in ewes allowing a deeper semen deposition. Therefore, semen doses may have fewer semen cells/ml. More doses can be set from each ejaculation. After been collected and assessed, semen may be preserved fresh, cooled or freeze/thawed. The main aim of this study was to compare the efficiency of AI with fresh or cooled semen in local Serrana goats. Twenty nine adult goats were inseminated with fresh semen (37ºC) and 28 with cooled semen (15ºC). Ovarian activity was controlled by a short-term progestogens treatment and ovulation was stimulated by eCG. Semen was collected by electroejaculation. After assessment semen samples were diluted with commercial extenders. Fresh semen was always kept at 37ºC. A refrigerated bath was used to cool diluted semen temperature from 37ºC to 15ºC (2 hours) – cooled semen. French straws were filled at 15ºC. Fresh or cooled semen doses had at least 80 x 106 cells/ml. AI was performed at a fixed time after eCG injection (43 ± 1 hour). Semen was deposited as deep as possible. Results are still been under analysis.
Artificial insemination (AI) is more common among goats than ewes. Better reproductive rates are to be expected between goats. Cervix anatomy is more suitable to AI in goats than in ewes allowing a deeper semen deposition. Therefore, semen doses may have fewer semen cells/ml. More doses can be set from each ejaculation. After been collected and assessed, semen may be preserved fresh, cooled or freeze/thawed. The main aim of this study was to compare the efficiency of AI with fresh or cooled semen in local Serrana goats. Twenty nine adult goats were inseminated with fresh semen (37ºC) and 28 with cooled semen (15ºC). Ovarian activity was controlled by a short-term progestogens treatment and ovulation was stimulated by eCG. Semen was collected by electroejaculation. After assessment semen samples were diluted with commercial extenders. Fresh semen was always kept at 37ºC. A refrigerated bath was used to cool diluted semen temperature from 37ºC to 15ºC (2 hours) – cooled semen. French straws were filled at 15ºC. Fresh or cooled semen doses had at least 80 x 106 cells/ml. AI was performed at a fixed time after eCG injection (43 ± 1 hour). Semen was deposited as deep as possible. Results are still been under analysis.
Description
Keywords
Cabra Serrana Inseminação artificial Sémen fresco Sémen refrigerado
Citation
Leão, Ana; Dendena, Marina; Mateus, Óscar; Correia, Teresa Montenegro; Maurício, Raimundo; Valentim, Ramiro (2017). Inseminação artificial em cabras da raça Serrana: sémen fresco ou refrigerado. In Martins, Anabela (Coord.) IV Encontro de Investigadores do Instituto Politécnico de Bragança: livro de resumos. Bragança: Instituto Politécnico. ISBN 978-972-745-218-7
Publisher
Instituto Politécnico de Bragança