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Discrimination of three bacteria species using a potentiometric electronic tongue

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dc.contributor.authorTeixeira, Guilherme Gobbi
dc.contributor.authorKatel, Anna
dc.contributor.authorVeloso, Ana C.A.
dc.contributor.authorDias, L.G.
dc.contributor.authorDias, Teresa
dc.contributor.authorPeres, António M.
dc.date.accessioned2020-02-26T11:39:30Z
dc.date.available2020-02-26T11:39:30Z
dc.date.issued2019
dc.description.abstractThe detection, monitoring and/or prevention of microorganism growing is of utmost relevance in several research fields, from food to environmental areas, being an important topic either from an academic or an industrial point of view. Conventional methods like plating techniques are the most widely used, being needed novel and faster screening methodologies like electronic noses, electronic tongue (E-tongue) and impedance based methods. In the present work, a potentiometric E-tongue (Fig.1), comprising 40 lipid polymeric sensor membranes with cross-sensitivity, was used to identify and discriminate three bacteria (Enterococcus faecalis ATCC29212; Staphylococcus aureus ATCC653 and Escherichia coli ATCC29998) at two concentrations levels (low and high). Brain Heart Infusion Broth medium was used for cultivating each of the three microorganisms, which were then individually inoculated into 1L Erlenmeyer flask (working volume of 300 mL) and incubated overnight (batch mode) at 35ºC, on a rotary shaker (90 rpm). After incubation, the biomass was spectrophotometrically determined, being measured the optical density at 550 nm. The cultures were split in volumes of 50 mL. The cells of each sample were harvested (centrifugation at 9000 rpm for 10 min) and stored at -20ºC. The obtained biomass was dried at 30ºC, and stored at -20ºC. Before E-tongue analysis, the cells were rehydrated with 20 mL of deionized water for 30 minutes at room temperature and aqueous sample solutions with different cell concentrations were obtained. Each E-tongue analysis took five minutes, enabling establishing a pseudo-equilibrium between the samples and the sensors´membranes, being the signals potentiometric profiles recorded. The classification performance of E-tongue was assessed by applying a linear discriminant analysis (LDA) coupled with the meta-heuristic simulated annealing (SA) variable selection algorithm. The preliminary results showed that E-tongue-LDA-SA predicting model could be established, based on the information gathered by a sub-set of 15 sensors, allowing to correctly classify (100%) (Fig.2) and 85% (original and leave-one-out cross-validation procedure, respectively) of the samples according to the microorganism and respectively concentration level.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTeixeira G.G., Katel A., Veloso A.C.A., Dias L.G., Dias T., Peres A.M. (2019). Discrimination of three bacteria species using a potentiometric electronic tongue. MicroBiotec19: Congress of Microbiology and Biotechnology: book of abstracts. Coimbrapt_PT
dc.identifier.urihttp://hdl.handle.net/10198/20683
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectE-tonguept_PT
dc.subjectBacteriapt_PT
dc.titleDiscrimination of three bacteria species using a potentiometric electronic tonguept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCoimbrapt_PT
oaire.citation.titleCONGRESS OF MICROBIOLOGY AND BIOTECHNOLOGY 2019pt_PT
person.familyNameTeixeira
person.familyNameDias
person.familyNameDias
person.familyNamePeres
person.givenNameGuilherme Gobbi
person.givenNameLuís G.
person.givenNameTeresa
person.givenNameAntónio M.
person.identifier142703
person.identifier107333
person.identifier.ciencia-id7416-7DC0-4FD6
person.identifier.ciencia-id2F11-9092-FAAF
person.identifier.ciencia-idCF16-5443-F420
person.identifier.orcid0000-0002-3734-1592
person.identifier.orcid0000-0002-1210-4259
person.identifier.orcid0000-0002-9419-9561
person.identifier.orcid0000-0001-6595-9165
person.identifier.ridI-8470-2012
person.identifier.scopus-author-id23569169900
person.identifier.scopus-author-id56830642600
person.identifier.scopus-author-id7102331969
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationcee38fc1-7f37-4784-b764-a790a095bce0
relation.isAuthorOfPublicationeac8c166-4056-4ed0-8d8d-7ecb2c4481a5
relation.isAuthorOfPublication87c9cae0-314d-4622-ba85-4d3ec5726606
relation.isAuthorOfPublication7d93be47-8dc4-4413-9304-5b978773d3bb
relation.isAuthorOfPublication.latestForDiscovery87c9cae0-314d-4622-ba85-4d3ec5726606

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