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Origins, diversity, and adaptive evolution of DWV in the honey bees of the Azores: the impact of the invasive mite Varroa destructor

2024Journal article Open access
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dc.contributor.authorLopes, Ana Rita
dc.contributor.authorLow, Matthew
dc.contributor.authorMartín-Hernández, Raquel
dc.contributor.authorPinto, M. Alice
dc.contributor.authorMiranda, Joachim
dc.date.accessioned2024-10-08T13:03:33Z
dc.date.available2024-10-08T13:03:33Z
dc.date.issued2024
dc.description.abstractDeformed wing virus (DWV) is a honey bee virus, whose emergence from relative obscurity is driven by the recent host-switch, adaptation, and global dispersal of the ectoparasitic mite Varroa destructor (a highly efficient vector of DWV) to reproduction on honey bees (Apis mellifera). Our study examines how varroa affects the continuing evolution of DWV, using the Azores archipelago, where varroa is present on only three out of the eight Islands, as a natural experimental system for comparing different evolutionary conditions and trajectories. We combined qPCR of 494 honey bee colonies sampled across the archipelago with amplicon deep sequencing to reveal how the DWV genetic landscape is altered by varroa. Two of the varroa-free Islands were also free of DWV, while a further two Islands were intriguingly dominated by the rare DWV-C major variant. The other four Islands, including the three varroa-infested Islands, were dominated by the common DWV-A and DWV-B variants. The varroa-infested Islands had, as expected, an elevated DWV prevalence relative to the uninfested Islands, but not elevated DWV loads, due the relatively high prevalence and loads of DWV-C on the varroa-free Islands. This establishes the Azores as a stable refuge for DWV-C and provides the most convincing evidence to date that at least some major strains of DWV may be capable of not just surviving, but actually thriving in honey bees in the absence of varroa-mediated transmission. We did not detect any change in DWV genetic diversity associated with island varroa status but did find a positive association of DWV diversity with virus load, irrespective of island varroa status.pt_PT
dc.description.sponsorshipFinancial support was provided by Portuguese funds through the Foundation for Science and Technology (FCT, Portugal) and by FEDER (Fundo Europeu de Desenvolvimento Regional) through the program COMPETE 2020-POCI (Programa Operacional para a Com-petividade e Internacionalização) in the framework of the project BEEHAPPY (POCI-01-0145-FEDER-029871). ARL was funded by FCT through the individual research grant SFRH/BD/143627/2019. JRM received additional financial support from the Onorati-Patriarca Foundation, the Swedish Research Council for Sus-tainable Development (FORMAS) grants 2017-00623, 2018-01142, and 2022-01462; the Swedish Research Council (VR) grant 2018-04772; the Oscar and Lili Lamms Memorial Foundation grant FO2020-0011; the Swedish Ministry of Agriculture National Pro-gramme for the Beekeeping Sector grants 2020-1819-2, 2021-1836, and 2021-279; and by FKASSAN. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021). ML was funded by the Swedish Research Council (2017-03963).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLopes, Ana R.; Low, Matthew; Martín-Hernández, Raquel; Pinto, M. Alice; De Miranda, Joachim R. (2024). Origins, diversity, and adaptive evolution of DWV in the honey bees of the Azores: the impact of the invasive mite Varroa destructor. Virus Evolution. ISSN 2057-1577. 10:1, p. 1-19pt_PT
dc.identifier.doi10.1093/ve/veae053pt_PT
dc.identifier.issn2057-1577
dc.identifier.urihttp://hdl.handle.net/10198/30369
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford Academicpt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectApis melliferapt_PT
dc.subjectVarroa destructorpt_PT
dc.subjectDeformed wing viruspt_PT
dc.subjectAzores archipelagopt_PT
dc.subjectASV genetic diversitypt_PT
dc.subjectVirus quasispeciespt_PT
dc.subjectInvasive vectorpt_PT
dc.titleOrigins, diversity, and adaptive evolution of DWV in the honey bees of the Azores: the impact of the invasive mite Varroa destructorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F143627%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.titleVirus Evolutionpt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLopes
person.familyNamePinto
person.givenNameAna
person.givenNameM. Alice
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-9663-8399
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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