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Species identification in heat processed meat products

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dc.contributor.authorSoares, Sónia
dc.contributor.authorAmaral, Joana S.
dc.contributor.authorMafra, Isabel
dc.contributor.authorOliveira, Beatriz
dc.date.accessioned2011-06-01T14:00:04Z
dc.date.available2011-06-01T14:00:04Z
dc.date.issued2009
dc.description.abstractIn this work, species-specific PCR techniques were developed and optimized to identify pork, poultry and cow species in Frankfurt sausages. For pork and poultry, which are the most frequently used meats in Frankfurters, reference binary samples (pork in poultry and poultry in pork) were prepared in the laboratory. In both cases, the established conditions allowed the detection of levels as low as 0.1% of added pork/poultry meat. Real-time PCR assays based on the measurement of fluorescence increments using SYBR Green I dye were used for quantitative purposes. Several samples were acquired in the retail market and tested for their label compliance. Results showed 3 mislabelled samples regarding cow species and 2 for poultry species. Although the qualitative results showed the undeclared presence of pork meat in 2 poultry sausages samples, quantitative results showed levels < 0.1%. These low levels are probably not related to fraudulent practices, but possibly due to cross-contaminations during industrial processing.por
dc.identifier.citationSoares, S.; Amaral, J.S.; Mafra, I.; Oliveira, M.B.P.P (2009). Species identification in heat processed meat products. In Actas do 9º Encontro de Química dos Alimentos. Angra do Heroísmo.por
dc.identifier.urihttp://hdl.handle.net/10198/4869
dc.language.isoporpor
dc.peerreviewedyespor
dc.subjectMeatpor
dc.subjectPorkpor
dc.subjectPoultrypor
dc.subjectCowpor
dc.subjectPCRpor
dc.titleSpecies identification in heat processed meat productspor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceAngra do Heroísmopor
oaire.citation.titleActas do 9º Encontro de Química dos Alimentospor
person.familyNameAmaral
person.givenNameJoana S.
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-3648-7303
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor
relation.isAuthorOfPublication42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery42be2cf4-adc4-4e7f-ac60-7aab515b38cd

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