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Agrocybe cylindracea bio-residues: a sustainable source of ergosterol-rich bioactive extracts

2019Conference object Open access
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dc.contributor.authorSilva, Ana Rita
dc.contributor.authorPinela, José
dc.contributor.authorCaleja, Cristina
dc.contributor.authorCosta, Cristina
dc.contributor.authorBarros, Joana
dc.contributor.authorFerreira, Inês
dc.contributor.authorNunes, João
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-06-19T14:45:43Z
dc.date.available2019-06-19T14:45:43Z
dc.date.issued2019
dc.description.abstractThe world production of edible mushrooms has increased more than 30‐fold since 1978 and, on average, each customer consumes 5 kg of mushrooms per year [1]. Depending on the dimension of the mushroom industry, the amount of obtained by-products may range from 20 to 35% in weight of fresh mushroom [2]. Indeed, 38% of the 90 million tonnes of food waste produced by the European Union every year has its origin in the food manufacturing sector [3]. However, there are several strategies of transforming these wastes into high value-added products based on the cascade use principle, and this is exactly what the MicoBioExtract project aims to perform. Thus, the bio-residues of a popular edible mushroom in southern Europe, Agrocybe cylindracea, were studied as a sustainable source of bioactive extracts [4]. Ergosterol has been reported to be one of the most important compounds, contributing to the health-promoting benefits, associated with mushrooms’ consumption [2]. Its extraction was performed using a heat-assisted technique and applying the response surface methodology, in order to optimize the combined effects of the variables time, temperature and solvent percentage, using a circumscribed central composite design with 16 independent combinations and 4 replicated centre points. At the optimum condition predicted by the model, the bioactivity of the extract was tested, evaluating the antioxidant (TBARS assay) and cytotoxic (in a porcine liver primary cell culture, PLP2) activities. The results obtained from the optimization study showed a significant interaction between temperature and extraction time, with an 8.24% extraction yield. The global optimum condition for ergosterol extraction predicted by the model was 150 min at 90 °C, with 329 mg of ergosterol per 100 g of dry weight sample. Regarding the bioactive potential, namely the antioxidant activity, this extract was capable of preventing the formation of malondialdehyde, a secondary product of lipid peroxidation in the TBARS assay. Concerning the cytotoxicity of the extracts against the PLP2 cell line, the results showed no significant cytotoxic effect, with GI50 values higher than 400 μg/mL. Thus, the extraction of molecules with a high nutritional and bioactive value from mushroom bio-residues and the goal to incorporate them in functional foods and nutraceuticals could boost the circular bio-economy, and help developing strategies towards promoting sustainability.pt_PT
dc.description.sponsorshipThe authors are grateful to FCT, Portugal and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and the research contracts of J. Pinela (Project AllNatt, POCI-01-0145-FEDER-030463) and L. Barros; this work was funded by FEDER through POCI, within the scope of project MicoBioExtract (POCI-01-0247-FEDER- 033939).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, Ana Rita; Pinela, José; Caleja, Cristina; Costa, Cristina; Barros, Joana; Ferreira, Inês; Nunes, João; Prieto, M.A.; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Agrocybe cylindracea bio-residues: a sustainable source of ergosterol-rich bioactive extracts. In XX EuroFoodChem Conference. Porto. ISBN 978-989-8124-26-5pt_PT
dc.identifier.isbn978-989-8124-26-5
dc.identifier.urihttp://hdl.handle.net/10198/19350
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAgrocybe cylindraceapt_PT
dc.titleAgrocybe cylindracea bio-residues: a sustainable source of ergosterol-rich bioactive extractspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlacePorto, Portugalpt_PT
oaire.citation.titleXX EuroFoodChem Conferencept_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSilva
person.familyNamePinela
person.familyNameCaleja
person.familyNamePrieto Lage
person.familyNameBarros
person.familyNameFerreira
person.givenNameAna Rita
person.givenNameJosé
person.givenNameCristina
person.givenNameMiguel A.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifierABD-5857-2020
person.identifier107688
person.identifier469085
person.identifier144781
person.identifier.ciencia-id0E1A-35C1-C79A
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person.identifier.ciencia-id221E-1E00-AB74
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-5819-9065
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-5769-8484
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridB-4466-2014
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id36514202500
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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