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Detection methods for aflatoxin M1 in dairy products

2020Journal article Open access
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dc.contributor.authorVaz, Andreia
dc.contributor.authorSilva, Ana C. Cabral
dc.contributor.authorRodrigues, Paula
dc.contributor.authorVenâncio, Armando
dc.date.accessioned2022-10-10T09:53:30Z
dc.date.available2022-10-10T09:53:30Z
dc.date.issued2020
dc.description.abstractMycotoxins are toxic compounds produced mainly by fungi of the genera Aspergillus, Fusarium and Penicillium. In the food chain, the original mycotoxin may be transformed in other toxic compounds, reaching the consumer. A good example is the occurrence of aflatoxin M1 (AFM1) in dairy products, which is due to the presence of aflatoxin B1 (AFB1) in the animal feed. Thus, milkbased foods, such as cheese and yogurts, may be contaminated with this toxin, which, although less toxic than AFB1, also exhibits hepatotoxic and carcinogenic effects and is relatively stable during pasteurization, storage and processing. For this reason, the establishment of allowed maximum limits in dairy products and the development of methodologies for its detection and quantification are of extreme importance. There are several methods for the detection of AFM1 in dairy products. Usually, the analytical procedures go through the following stages: sampling, extraction, clean‐up, determination and quantification. For the extraction stage, the use of organic solvents (as acetonitrile and methanol) is still the most common, but recent advances include the use of the Quick, Easy, Cheap, Effective, Rugged, and Safe method (QuEChERS) and proteolytic enzymes, which have been demonstrated to be good alternatives. For the clean‐up stage, the high selectivity of immunoaffinity columns is still a good option, but alternative and cheaper techniques are becoming more competitive. Regarding quantification of the toxin, screening strategies include the use of the enzyme‐linked immunosorbent assay (ELISA) to select presumptive positive samples from a wider range of samples, and more reliable methods—high performance liquid chromatography with fluorescence detection or mass spectroscopy—for the separation, identification and quantification of the toxin.pt_PT
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE‐01‐0145‐FEDER‐ 000004) funded by the European Regional Development Fund under the scope of Norte2020 ‐ Programa Operacional Regional do Norte. PR is grateful to FCT and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019). We would like to thank the Foundation for Science and Technology (FCT) for the Ph.D. scholarship given to Andreia Vaz (SFRH/BD/129775/2017).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVaz, Andreia; Silva, Ana C. Cabral; Rodrigues, Paula; Venâncio, Armando (2020). Detection methods for aflatoxin M1 in dairy products. Microorganisms. eISSN 2076-2607. 8:2, p. 1-16pt_PT
dc.identifier.doi10.3390/microorganisms8020246pt_PT
dc.identifier.eissn2076-2607
dc.identifier.urihttp://hdl.handle.net/10198/25968
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relationMountain Research Centre
dc.relationAflatoxin M1 in dairy products: causes and strategies to overcome it
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAflatoxin M1pt_PT
dc.subjectQuantificationpt_PT
dc.subjectMilkpt_PT
dc.subjectDairy productspt_PT
dc.subjectAnalytical techniquespt_PT
dc.titleDetection methods for aflatoxin M1 in dairy productspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardTitleMountain Research Centre
oaire.awardTitleAflatoxin M1 in dairy products: causes and strategies to overcome it
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F129775%2F2017/PT
oaire.citation.issue2pt_PT
oaire.citation.startPage246pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameRodrigues
person.givenNamePaula
person.identifier.ciencia-id2312-18AE-DA42
person.identifier.orcid0000-0002-3789-2730
person.identifier.scopus-author-id7102639646
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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