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HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species

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dc.contributor.authorSilveira, Tayse F. F. da
dc.contributor.authorRodrigues, Daniele Bobrowski
dc.contributor.authorVazquez, A.P.
dc.contributor.authorAlvarez, Paula Barciela
dc.contributor.authorCarpena Rodríguez, María
dc.contributor.authorSimal-Gandara, Jesus
dc.contributor.authorPrieto Lage, Miguel A.
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-03-17T14:01:58Z
dc.date.available2023-03-17T14:01:58Z
dc.date.issued2022
dc.description.abstractThe genus Euphorbia comprises more than 2,000 species widely distributed in Asia, Africa and Latin America. They have been extensively used in folk medicine to treat disorders such as abdominal pain, skin diseases, tumors, wart, among others¹. Although the well-recognized ethnopharmacological relevance of Euphorbia species, most of them have not been studied yet, such as Euphorbia hirta and Euphorbia jokinii. In this context, screening the bioactive molecules potentially responsible for the observed medicinal effects is an initial, but key step for discovering novel active compounds and understanding the mechanisms of action underlying their biological activities. Thus, this study aimed at determining the phenolic compounds composition of E. Hirta and E. jokinii. Both plants were freezedried, milled, and extracted with ethanol:water (60:40 v/v) for 3 hours at 45 °C. After centrifugation, the extracts were freeze-dried, re-suspended in ethanol:water (20:80 v/v), filtered and injected into the HPLC-DAD-(ESI)-MS/MS system. The phenolic profile of E. Hirta and E. Jokinii revealed mainly the presence of flavonoids and galloyl derivatives. Thirty-two compounds were tentatively identified in E. Hirta and twenty-two in E. Jokinii. Quercetin 3-O-rhamnoside was the major compound in both species, with E. Hirta showing the highest content (10.5 mg/g vs 8.2 mg/g extract). Our results indicate that E. Hirta and E. Jokinii, yet underexploited Euphorbia species, are interesting sources of flavonoids. Further studies should evaluate the relationship between these compounds and the biological activities of these plants, as well as establish possible related mechanisms of action.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021). L. Barros also thanks FCT through the institutional scientific employment program contract for her contract. T. Silveira, thanks to Fundação BPI La - - Rodrigues acknowledges the Fundo Europeu de Desenvolvimento Regional (FEDER) and the NORTE2020 Programme within the project POCI-01-0247-FEDER-113508). The research leading to these results was supported by MICINN supporting the Ramón y Cajal grant for M.A. Prieto (RYC-2017-22891); by Xunta de Galicia for supporting the program EXCELENCIA-ED431F 2020/12 and the pre-doctoral grant of M. Carpena (ED481A 2021/313).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilveira, Tayse Ferreira; Rodrigues, Daniele B.; Vazquez, A.P.; Alvarez, P.B.; Carpena Rodríguez, María; Simal-Gandara, Jesus; Prieto Lage, Miguel A.; Barros, Lillian (2022). HPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia species. In Livro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA. Aveiropt_PT
dc.identifier.isbn978-989-8124-37-1
dc.identifier.urihttp://hdl.handle.net/10198/27811
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Portuguesa de Químicapt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMetabolic fingerprintingpt_PT
dc.subjectMedicinal herbspt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleHPLC-DAD-(ESI)-MS/MS analysis as the first step to metabolic fingerprinting of medicinal herbs: the case of underexploited Euphorbia speciespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDP/00690/2020
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.conferencePlaceAveiropt_PT
oaire.citation.endPage195pt_PT
oaire.citation.startPage195pt_PT
oaire.citation.titleLivro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPApt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSilveira
person.familyNameRodrigues
person.familyNamePrieto
person.familyNameBarros
person.givenNameTayse F. F. da
person.givenNameDaniele Bobrowski
person.givenNameMiguel A.
person.givenNameLillian
person.identifier107688
person.identifier469085
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person.identifier.ciencia-idA91B-FE1D-B275
person.identifier.ciencia-id221E-1E00-AB74
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0003-0260-5751
person.identifier.orcid0000-0002-3513-0054
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridG-4516-2011
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id35937495700
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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