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Phenolic compounds and antioxidant and cytotoxic properties of maritime pine (Pinus pinaster Ait.) bark extracts

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dc.contributor.authorAlonso-Esteban, José Ignacio
dc.contributor.authorFinimundy, Tiane C.
dc.contributor.authorPereira, Carla
dc.contributor.authorVelho, Manuela Vaz
dc.contributor.authorVaz, Josiana A.
dc.contributor.authorCarocho, Márcio
dc.contributor.authorBarros, Lillian
dc.date.accessioned2023-03-15T14:36:33Z
dc.date.available2023-03-15T14:36:33Z
dc.date.issued2022
dc.description.abstractMaritime pine (Pinus pinaster Ait.) is a pine species native to the western Mediterranean basin which has an economic importance in southwestern Europe due to the industrial uses of its wood. Other parts of the tree, including the bark, are considered by-products, and they stand out because of the presence of secondary metabolites, such as phenolic compounds. These compounds have biological potential, making them suitable to produce food supplements and functional foods, among other applications [1]. Two extracts were obtained from maritime pine bark by microwave assisted extraction (MAE) and ultrasound assisted extraction (UAE), and their phenolic compound content and antioxidant and cytotoxic properties were analysed. Phenolic compounds analysis was carried out by HPLD-DADESI/ MS [2]. Antioxidant properties were measured via thiobarbituric acid reactive substances (TBARS) formation inhibition method in brain cell homogenates, cellular antioxidant activity (CAA) in murine macrophage cells RAW 246.7, and oxidative haemolysis inhibition assay (OxHLIA) in sheep blood erythrocytes [2,3]. The cytotoxic properties of the extracts were screened in tumour and non-tumour cells by the sulforhodamine B assay [3]. The MAE showed a greater yield of extraction, three times higher than UAE. Major phenolic compounds in maritime pine extracts were catechin and B-type procyanidins, and some A-type procyanidins and taxifolin derivatives were also found in both extracts. The best antioxidant activity results were observed in the TBARS method and OxHLIA, especially in the case of the extract obtained by MAE, although it did not showed CAA at the maximum concentration studied (2 mg/mL), unlike the extract obtained by UAE, which showed some activity at that concentration. Regarding cytotoxic properties, the two extracts showed similar results since they presented activity in tumour and nontumour lines with IG50 values higher than those observed with the positive control (ellipticine). Therefore, MAE was a better extraction method for obtaining a functional extract from maritime pine bark.pt_PT
dc.description.sponsorshipThe authors are grateful to FCT (Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021); M. Carocho (CEECIND/00831/2018), L. Barros and C. Pereira thank FCT for their Individual and Institutional Employment Program- Contracts. J. I. Alonso-Esteban thanks the Ministry of Universities (Spain) and the European Union-Next Generation EU Funds for the Margarita Salas grant (UCM/572725/2021). This work was financed by the European Regional Development Fund (ERDF) through the Regional Operation Program North 2020, within the scope of the R&D Enterprise Copromotion project (NORTE-01-0247-FEDER-069584).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlonso-Esteban, José Ignacio; Finimundy, Tiane C.; Pereira, Carla; Velho, Manuela Vaz; Vaz, Josiana A.; Carocho, Márcio; Barros, Lillian (2022). Phenolic compounds and antioxidant and cytotoxic properties of maritime pine (Pinus pinaster Ait.) bark extracts. In XXVI Encontro Galego-Portugues de Química: Book of Abstracts. Santiago de Compostelapt_PT
dc.identifier.isbn978-84-09-45895-0
dc.identifier.urihttp://hdl.handle.net/10198/27743
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversidade de Santiago de Compostelapt_PT
dc.relationLA/P/0007/2021pt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationNORTE-01-0247-FEDER-069584pt_PT
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMaritime pinept_PT
dc.subjectPinus pinaster Ait.pt_PT
dc.subjectResearch Subject Categories::MEDICINE::Morphology, cell biology, pathologypt_PT
dc.titlePhenolic compounds and antioxidant and cytotoxic properties of maritime pine (Pinus pinaster Ait.) bark extractspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDP/00690/2020
oaire.awardNumberCEECIND/00831/2018/CP1578/CT0001
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2018/CEECIND%2F00831%2F2018%2FCP1578%2FCT0001/PT
oaire.citation.conferencePlaceSantiago de Compostelapt_PT
oaire.citation.endPage349pt_PT
oaire.citation.startPage349pt_PT
oaire.citation.titleXXVI Encontro Galego-Portugues de Química: Book of Abstractspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2018
person.familyNameFinimundy
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person.familyNameCarocho
person.familyNameBarros
person.givenNameTiane C.
person.givenNameCarla
person.givenNameJosiana A.
person.givenNameMárcio
person.givenNameLillian
person.identifier1415151
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person.identifier.orcid0000-0003-0093-771X
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0002-8978-4547
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridK-1629-2016
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
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