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Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice

2022Journal article Open access
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dc.contributor.authorRocha, Paola dos Santos
dc.contributor.authorOrué, Sarah Lam
dc.contributor.authorLeite, Daniel Ferreira
dc.contributor.authorEspindola, Priscilla Pereira de Toledo
dc.contributor.authorCassemiro, Nadla Soares
dc.contributor.authorSilva, Denise Brentan
dc.contributor.authorCarollo, Carlos Alexandre
dc.contributor.authorNunes-Souza, Valéria
dc.contributor.authorRabelo, Luiza Antas
dc.contributor.authorCampos, Jaqueline Ferreira
dc.contributor.authorEstevinho, Leticia M.
dc.contributor.authorSantos, Edson Lucas dos
dc.date.accessioned2022-11-04T10:38:05Z
dc.date.available2022-11-04T10:38:05Z
dc.date.issued2022
dc.description.abstractObesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRocha, Paola dos Santos da; Orué, Sarah Lam; Leite, Daniel Ferreira; Espindola, Priscilla Pereira de Toledo; Cassemiro, Nadla Soares; Silva, Denise Brentan da; Carollo, Carlos Alexandre; Nunes-Souza, Valéria; Rabelo, Luiza Antas; Campos, Jaqueline Ferreira; Estevinho, Leticia M.; Santos, Edson Lucas dos. (2022). Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice. Oxidative Medicine and Cellular Longevity. ISSN 19420900. 2022, p. 1-13pt_PT
dc.identifier.issn19420900
dc.identifier.urihttp://hdl.handle.net/10198/26067
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherHindawipt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidantspt_PT
dc.subjectBloodpt_PT
dc.subjectFlavonoidspt_PT
dc.subjectQuality Controlpt_PT
dc.titleBeneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 micept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleOxidative Medicine and Cellular Longevitypt_PT
person.familyNameEstevinho
person.givenNameLetícia M.
person.identifier.ciencia-idBA14-09D6-A406
person.identifier.orcid0000-0002-9249-1948
person.identifier.scopus-author-id6506577664
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4a1d5ba2-1854-4ca5-89a4-73f35e964df9
relation.isAuthorOfPublication.latestForDiscovery4a1d5ba2-1854-4ca5-89a4-73f35e964df9

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