Logo do repositório
 
Publicação

Exploring the inhibitory potential of 2-styrylchromones on pancreatic α- amylase

2023Póster em conferência Acesso aberto
Ver registo simples
datacite.subject.fosEngenharia e Tecnologia::Biotecnologia Industrial
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorSantos, Clementina M.M.
dc.contributor.authorProença, Carina
dc.contributor.authorFreitas, Marisa
dc.contributor.authorAraújo, Alberto N.
dc.contributor.authorSilva, Artur M.S.
dc.contributor.authorFernandes, Eduarda
dc.contributor.editorXVIIIENSPQ
dc.date.accessioned2026-05-14T15:02:24Z
dc.date.available2026-05-14T15:02:24Z
dc.date.issued2023
dc.description.abstractDiabetes mellitus is a metabolic disorder that afflicts about 537 million people worldwide and this number is predicted to rise to 643 million by 2030, according to the International Diabetes Federation.1 It is characterized by hyperglycemia, caused by the deficiency in the secretion of insulin and/or in the action of this pancreatic hormone. To date, the best therapeutic strategy known consists of inhibiting carbohydrate-hydrolyzing enzymes, namely the α-amylase enzyme. The currently marketed inhibitors (e.g., acarbose, miglitol, and voglibose) are based on carbohydrate-related structures, with moderate affinity for the enzyme and with disturbing side effects.2 Thus, an active pursuit for novel and more effective anti-diabetic drugs has been carried out and a wide variety of structurally diverse heterocyclic compounds has been studied. Chromones are among the oxygenated 6-membered heterocycles evaluated and the results exhibited by some 2-arylchromones point out the relevance of this class of compounds in the inhibition of α‐amylase enzymatic activity.3 Nonetheless, a detailed investigation of the effects of the restricted group of chromones known as 2-styrylchromones (2-SC) has not been conducted to date. With this rationale in mind and as part of our on-going project, the aim of the present study is to investigate the effect of a panel of twelve 2-SC 1 on pancreatic α-amylase activity and their mechanism of inhibition, to infer about the importance of this class of compounds in the management of type 2 diabetes and its complications. α-Amylase was exposed to different concentrations of 2-SC 1 and the hydrolysis of the substrate 2-chloro-p-nitrophenyl-α-D-maltotriose was monitored spectrophotometrically at 405 nm. Acarbose was used as the standard inhibitor. In addition, the study of the inhibition type was carried out through nonlinear regression Michaelis-Menten enzymatic kinetics and the corresponding Lineweaver-Burk plot.4 The results showed that the IC50 values obtained ranged from 26 to 174 μM, considerably higher than the positive control acarbose (IC50 = 0.62 ± 0.07 μM). All active compounds revealed a competitive type of inhibition while for the positive control a mixed type of inhibition was obtained. More details concerning the structure-activity relationship will be presented and discussed in this communication.eng
dc.description.sponsorshipThiswork receivedsupport fromnational funds (FCT/MCTES, FundaçãoparaaCiênciae TecnologiaandMinistériodaCiência,TecnologiaeEnsinoSuperior) throughtheprojectsof CIMO (UIDB/00690/2020 and UIDP/00690/2020), SusTEC (LA/P/0007/2021), REQUIMTE (UIDB/50006/2020andUIDP/50006/2020)andthroughtheprojectwiththereferenceEXPL/MEDQUI/0815/2021. Carina Proença acknowledges funding from FCT through the project, EXPL/MEDQUI/0815/2021. MarisaFreitasacknowledgeshercontractundertheScientificEmploymentStimulus-IndividualCall(CEECIndividual)2020.04126.CEECIND/CP159CT0006andLAQV-REQUIMTEforhercontractunderthereferenceLA/P/0008/2020.
dc.identifier.citationSantos, Clementina M.M.; Proença, Carina; Freitas, Marisa; Araújo, Alberto N.; Silva, Artur M.S.; Fernandes, Eduarda (2023). Exploring the inhibitory potential of 2-styrylchromones on pancreatic α- amylase. In XVIII National Meeting of the Portuguese Society of Chemistry. Aveiro
dc.identifier.urihttp://hdl.handle.net/10198/36667
dc.language.isoeng
dc.peerreviewedyes
dc.relationMountain Research Center - UIDB/00690/2020
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationUIDP/50006/2020
dc.rights.uriN/A
dc.subject2-styrylchromones
dc.subjectPancreatic α-amylase
dc.subjectEnzymatic inhibition
dc.subjectDiabetes mellitus
dc.subjectChromones
dc.titleExploring the inhibitory potential of 2-styrylchromones on pancreatic α- amylaseeng
dc.typeconference poster
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDB/50006/2020
oaire.awardTitleMountain Research Center - UIDB/00690/2020
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.conferenceDate2023
oaire.citation.conferencePlaceAveiro
oaire.citation.titleXVIII Encontro Nacional da SPQ
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameSantos
person.givenNameClementina M.M.
person.identifier.ciencia-id9018-DB9C-C590
person.identifier.orcid0000-0003-4380-7990
person.identifier.scopus-author-id7201458663
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication64f662b6-775d-4ea0-bfd7-f527af0ac1a0
relation.isAuthorOfPublication.latestForDiscovery64f662b6-775d-4ea0-bfd7-f527af0ac1a0
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication0d71ca47-dd95-4204-8a34-d28377654df3
relation.isProjectOfPublication.latestForDiscovery29718e93-4989-42bb-bcbc-4daff3870b25

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Exploring the inhibitory .pdf
Tamanho:
1.59 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.75 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIMO - Posters em Encontros Científicos Nacionais