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Histological lesions in hpv16-transgenic model: the effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav.

2021Conference poster Open access
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dc.contributor.authorFerreira, Tiago
dc.contributor.authorNascimento-Gonçalves, Elisabete
dc.contributor.authorMoutinho, Magda S.S.
dc.contributor.authorPires, Maria João
dc.contributor.authorBastos, Margarida M.S.M.
dc.contributor.authorMedeiros, Rui
dc.contributor.authorNogueira, António José M.
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorCosta, Rui Miguel Gil
dc.contributor.authorRosa, Eduardo
dc.contributor.authorOliveira, Paula A.
dc.date.accessioned2023-03-16T11:55:35Z
dc.date.available2023-03-16T11:55:35Z
dc.date.issued2021
dc.description.abstractThe K14HPV16 mice is a skin squamous carcinoma model that can be used to test antitumoral properties of several chemical and natural compounds1. Lavandula penduculata (Mill.) Cav., known as lavender, belongs to the Laminaceae family and has been used in traditional medicine as infusions to treat several conditions2. This work aimed to evaluate the effects of the hydroethanolic French lavender extract (FLE) in an HPV16-transgenic mice model lesions. The extract was obtained through a maceration with ethanol/water (80:20, v/v) and its phenolic composition was determined by HPLC-DADESI/ MS. The FLE was dissolved in drinking water at 6.8 mg/10mL/animal and the animals were supplemented during 29 consecutive days. Twentyeight male mice were randomly divided into four groups: (n=7/group): group I (HPV16- control); II (HPV16- FLE); III (HPV16+ control) and IV (HPV16+ FLE). After 29 days all animals were sacrificed by xylazineketamine overdose following cardiac puncture to obtain blood samples. Skin samples (chest and ear), kidney, liver and spleen were processed for histological analysis.A total of thirteen compounds were identified in the hydroethanolic extract, being salvianolic acid B and rosmarinic acid the main molecules present. Moreover, the compounds revealed to be stable in the drinking water for 5 days. Histological analyses of skin samples from wild-type mice exposed (group II) and not exposed (group I) to FLE showed normal skin histology. Group III showed skin chest epidermal hyperplasia in 100% of the mice while group IV showed less epidermal hyperplasia frequency (66.6%) (p>0.05). Concerning to liver, kidney, and spleen lesions there are no differences between groups (p>0.05). The lavender extract did not prevent the progression of HPV-16 induced cutaneous lesions in this model. These data deserve more investigation to clarify the effect of lavender extract on HPV-16 lesions.pt_PT
dc.description.sponsorshipThis work was supported by European Investment Funds by FEDER/ COMPETE/POCI - Operational Competitiveness and Internationalization Program and National Funds by FCT - Portuguese Foundation for Science and Technology, under the projects Project UIDB/04033/2020 (CITAB), and PhD fellowship SFRH/BD/136747/2018 and 2020.04789.BD. The authors are also grateful to FCT for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/2020). L. Barros thanks FCT, P.I for her contract through the institutional scientific employment program. This work was also funded by the European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project GreenHealth - Norte-01-0145-FEDER-000042. The authors would like to thank BPGV for the samples provided.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, Tiago; Nascimento-Gonçalves, Elisabete; Moutinho, Magda S.S.; Pires, Maria João; Bastos, Margarida M.S.M.; Medeiros, Rui; Nogueira, António José M.; Barros, Lillian; Ferreira, Isabel C.F.R.; Costa, Rui Miguel Gil; Rosa, Eduardo; Oliveira, Paula A. (2021). Histological lesions in hpv16-transgenic model: the effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav. In III Jornadas Ibéricas de Toxicologia. Lisboapt_PT
dc.identifier.isbn978-989-654-772-1
dc.identifier.urihttp://hdl.handle.net/10198/27781
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversidade da Beira Interiorpt_PT
dc.relationNorte-01-0145-FEDER-000042pt_PT
dc.relationalterado para: “Contribuição para o estudo do cancro da próstata: influência do estilo de vida num modelo animal Contribution to the study of prostate cancer: lifestyle influence in an animal model”. Estudo do impacto da atividade física no microambiente do cancro da próstata num modelo animal
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationDevelop a spheroid 3D tumor model for drug screening in canine triple-negative mammary cancer
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLavandula penduculata (Mill.)pt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Bioengineeringpt_PT
dc.titleHistological lesions in hpv16-transgenic model: the effect of hidroethanolic extract of Lavandula pedunculata (mill.) Cav.pt_PT
dc.typeconference poster
dspace.entity.typePublication
oaire.awardTitlealterado para: “Contribuição para o estudo do cancro da próstata: influência do estilo de vida num modelo animal Contribution to the study of prostate cancer: lifestyle influence in an animal model”. Estudo do impacto da atividade física no microambiente do cancro da próstata num modelo animal
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardTitleDevelop a spheroid 3D tumor model for drug screening in canine triple-negative mammary cancer
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F136747%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.04789.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.citation.conferencePlaceLisboapt_PT
oaire.citation.titleIII Jornadas Ibéricas de Toxicologiapt_PT
oaire.fundingStreamFARH
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameNogueira
person.familyNameBarros
person.familyNameFerreira
person.givenNameAntónio José M.
person.givenNameLillian
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8A11-794E-36BD
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-8912-7355
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridJ-3600-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id55865568268
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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