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Desde os tempos antigos, o ser humano recorreu sempre aos produtos naturais como principal fonte primordial para a prevenção e tratamento de várias doenças. Estudos mostram que, nos países em desenvolvimento, 80% da população mundial recorre à medicina tradicional por ser considerada mais acessível, segura, eficaz e por apresentar menos reações adversas.
Em África, Zanha golungensis é utilizada tanto para fins alimentares como medicinais, nomeadamente no tratamento de várias enfermidades, como a malária, diarreia, dores de dentes, entorses, problemas respiratórios. O objectivo deste trabalho centra-se na caracterização do perfil químico e avaliação da atividade biológica do extrato da raiz da Z. golungensis, usada no tratamento da asma, segundo os praticantes da medicina tradicional em Nampula, na zona norte de Moçambique.
Para a caracterização dos compostos fenólicos foi usado o método HPLC-DAD-ESI/MS, onde foram identificados três compostos fenólicos com a mesma estrutura principal de crisoeriol, nomeadamente Crisoeriol-rhamnosyl-dirutinosideo, crisoeriol-dirhamnosyl-rutinosideo e crisoeriol-rhamnosyl-rutinosideo.
Verificou-se que o extrato apresentou atividade com a CMI de 10 mg/mL frente as bactérias Gram-negativo de origem clínica, e de origem alimentar, com CMI de 5mg/mL frente a Escherichia coli e CMI de 10mg/mL frente Pseudomona aeruginosa e Salmonella enterocolítica.
No que respeita à capacidade anti-inflamatória, o extrato apresentou um IC50 de 231,76±8,44 μg/mL, inibindo a produção de óxido nítrico (NO). Em relação à atividade antiproliferativa, o extrato demonstrou capacidade de inibir a proliferação de todas as linhas celulares tumorais em estudo. Contudo, o extrato não apresentou atividade antiproliferativa frente as linhas celulares não tumorais Vero.
No que concerne à atividade antioxidante, o extrato revelou capacidade de inibir a formação de substâncias reativas ao ácido tiobarbitúrico.
Os resultados obtidos indicam que a raiz da Z. golungensis possui atividade biológicas, o que comprova os seus efeitos benéficos no contexto da medicina tradicional em Moçambique.
Since ancient times, human beings have resorted to natural products as the primary source for the prevention and treatment of various diseases. Studies show that, in developing countries, 80% of the world's population resorts to traditional medicine because it is considered more accessible, safe, effective and presents fewer adverse reactions. In Africa, Zanha golungensis is used for both food and medicinal purposes, particularly in treating various illnesses, such as malaria, diarrhea, toothaches, sprains, and respiratory problems. The objective of this work focuses on characterizing the chemical profile and evaluating the biological activity of Z. golungensis root extract, used to treat asthma, according to traditional medicine practitioners in Nampula, in the north of Mozambique. For the characterization of phenolic compounds, the HPLC-DAD-ESI/MS method was used, where three phenolic compounds with the same main structure of chrysoeriol were identified, namely Chrysoeriol-rhamnosyl-dirutinoside, chrysoeriol-dirhamnosyl-rutinoside and chrysoeriol- rhamnosyl-rutinoside. It was found that the extract showed activity with a MIC of 10 mg/mL against Gram- negative bacteria of clinical origin and food origin, with a MIC of 5 mg/mL against Escherichia coli and a MIC of 10 mg/mL against Pseudomona aeruginosa and enterocolitic Salmonella. Regarding anti-inflammatory capacity, the extract presented an IC50 of 231.76±8.44 μg/mL, inhibiting the production of nitric oxide (NO). Regarding antiproliferative activity, the extract demonstrated the ability to inhibit the proliferation of all tumor cell lines under study. However, the extract did not show antiproliferative activity against Vero non-tumor cell lines. Regarding antioxidant activity, the extract revealed the ability to inhibit the formation of substances reactive to thiobarbituric acid. The results obtained indicate that the root of Z. golungensis has biological activity, which proves its beneficial effects in the context of traditional medicine in Mozambique.
Since ancient times, human beings have resorted to natural products as the primary source for the prevention and treatment of various diseases. Studies show that, in developing countries, 80% of the world's population resorts to traditional medicine because it is considered more accessible, safe, effective and presents fewer adverse reactions. In Africa, Zanha golungensis is used for both food and medicinal purposes, particularly in treating various illnesses, such as malaria, diarrhea, toothaches, sprains, and respiratory problems. The objective of this work focuses on characterizing the chemical profile and evaluating the biological activity of Z. golungensis root extract, used to treat asthma, according to traditional medicine practitioners in Nampula, in the north of Mozambique. For the characterization of phenolic compounds, the HPLC-DAD-ESI/MS method was used, where three phenolic compounds with the same main structure of chrysoeriol were identified, namely Chrysoeriol-rhamnosyl-dirutinoside, chrysoeriol-dirhamnosyl-rutinoside and chrysoeriol- rhamnosyl-rutinoside. It was found that the extract showed activity with a MIC of 10 mg/mL against Gram- negative bacteria of clinical origin and food origin, with a MIC of 5 mg/mL against Escherichia coli and a MIC of 10 mg/mL against Pseudomona aeruginosa and enterocolitic Salmonella. Regarding anti-inflammatory capacity, the extract presented an IC50 of 231.76±8.44 μg/mL, inhibiting the production of nitric oxide (NO). Regarding antiproliferative activity, the extract demonstrated the ability to inhibit the proliferation of all tumor cell lines under study. However, the extract did not show antiproliferative activity against Vero non-tumor cell lines. Regarding antioxidant activity, the extract revealed the ability to inhibit the formation of substances reactive to thiobarbituric acid. The results obtained indicate that the root of Z. golungensis has biological activity, which proves its beneficial effects in the context of traditional medicine in Mozambique.
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Zanha golungensis Caracterização química