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Advisor(s)
Abstract(s)
Angiogenesis is a biological process through which there is the formation of new blood vessels from preexisting
ones [I]. However, in pathological cases, the abnormal growth of new blood vessels promotes the
development of various diseases including cancer [2) through the production of atypically large amounts
of angiogenesis factors, e.g. the vascular endothelial growth factor (VEGF) [3]. The plant secondary
metabolites have been the subject of several studies to evaluate their benefits to human health. In
particular, the phenolic compounds have high potential for use in the food industry, including the
development of functional foods. Among these, apigenin has been associated with chemopreventive
effects related to cancer [4]. In fact, chemoprevention is a present-day concept and contemplates the use of
medicines, biological compounds or nutrients as an intervention strategy of cancer prevention.
In this work, an Arenaria montana L hydroethanolic extract was prepared and after characterization by
HPLC-DAD-ESI/MS showed to be rich in apigenin derivatives. Furthermore, it exhibited ability to inhibit
the phosphorylation of VEGFR-2 (vascular endothelium growth factor receptor) through an enzymatic
assay. However, for the major protection of bioactive compounds, the extract was microencapsulated by
an atomization/coagulation technique with alginate as the matrix material. Posteriorly, the hydroethanolic
extract, in free and microencapsulated forms, was incorporated in yogurts in order to develop a novel
chemopreventer food in relation to the angiogenesis process.
The functionalized yogurts with A. montana extracts (free and microencapsulated) showed a nutritional
value similar to the used control (yogurt without extract); however, the samples enriched with extracts
revealed added-value regarding the VEGFR-2 phosphorylation inhibition ability. This effect was more
effectively preserved over time in the samples functionalized with the protected extract.
Overall, this work contributes to the valorization of plants rich in flavonoids, exploring its antiangiogenic
potential with VEGFR-2 as target. Moreover, the atomization/coagulation technique allowed the
production of viable microspheres enriched with the plant extract. The microspheres were effectively
incorporated into yogurts, protecting the extract thus envisaging the development of novel functional
foods with chemopreventive effects.
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Citation
Ribeiro, Andreia; Oliveira, Franciely; Calhelha, Ricardo C.; Junior, Bogdan D.; Barreiro, M.F.; Ferreira, Isabel C.F.R. (2016). Antiangiogenic potential of yogurts added with extracts rich in apigenin derivatives. In 5° Encontro Português de Jovens Químicos (PYCheM) e o 1° Encontro Europeu de Jovens Químicos (EYCHeM). Guimarães