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Escherichia coli 0157:H7 is a foodborne pathogen classified as an Enterohemorrhagic E. coli being associated to foodborne outbreaks with high mortality. Since the traditional methods for its detection are often time-consuming, there is a need to develop new techniques that allow rapid, simple, reliable, specific and sensitive detection for a quick and effective medical intervention, as well as to ensure food safety. In the last years different approaches have bee suggested for this purpose including the use of biosensors based on different bioreceptors such enzymes, antibodies and DNA. Recently, there has been a growing interest regarding DNA biosensors due to advantages such as DNA high specificity and stability and the possibility developing devices that can be reusable after thermal melting of the DNA duplex [1].
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Rodrigues, R.; Pérez-Lorenzo, E.; Arana, S.; Amaral, J.S.; Rodrigues, P.S.; Mujika, M. (2012). Development and optimization of a biological protocol for DNA detection of escherichia coli O157:H7 by quartz crystal microbalance (QCM). In Livro de resumos do 11º Encontro de Química dos Alimentos. Bragança, Portugal