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Integrative phylogenetic, phylogeographic and morphological characterisation of the Unio crassus species complex reveals cryptic diversity with important conservation implications

2024Journal article Open access
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dc.contributor.authorLopes-Lima, Manuel
dc.contributor.authorGeist, Juergen P.
dc.contributor.authorEgg, S.
dc.contributor.authorBeran, Luboš
dc.contributor.authorBikashvili, Ani
dc.contributor.authorVan Bocxlaer, Bert
dc.contributor.authorBogan, Arthur E.
dc.contributor.authorBolotov, Ivan N.
dc.contributor.authorChelpanovskaya, O.A.
dc.contributor.authorDouda, Karel
dc.contributor.authorFernandes, Verónica
dc.contributor.authorGomes-dos-Santos, André
dc.contributor.authorGonçalves, Duarte Vasconcelos
dc.contributor.authorGürlek, Mustafa Emre
dc.contributor.authorJohnson, Nathan A.
dc.contributor.authorKaraouzas, Ioannis D.
dc.contributor.authorKebapçı, Ümit
dc.contributor.authorKondakov, Duarte Vasconcelos
dc.contributor.authorKuehn, Ralf
dc.contributor.authorLajtner, Jasna
dc.contributor.authorMumladze, Levan
dc.contributor.authorNagel, Karl Otto
dc.contributor.authorNeubert, Eike
dc.contributor.authorÖsterling, Martin E.
dc.contributor.authorPfeiffer, John M.
dc.contributor.authorPrié, Vincent
dc.contributor.authorRiccardi, Nicoletta
dc.contributor.authorSell, Jerzy
dc.contributor.authorSchneider, Lea D.
dc.contributor.authorShumka, Spase
dc.contributor.authorSîrbu, Ioan
dc.contributor.authorSkujienė, Grita
dc.contributor.authorSmith, Chase H.
dc.contributor.authorSousa, Ronaldo Gomes
dc.contributor.authorStöckl, K.
dc.contributor.authorTaskinen, Jouni K.
dc.contributor.authorTeixeira, Amílcar
dc.contributor.authorTodorov, Milcho T.
dc.contributor.authorTrichkova, Teodora A.
dc.contributor.authorUrbańska, Maria
dc.contributor.authorVälilä, Santtu
dc.contributor.authorVarandas, Simone Da Graça Pinto
dc.contributor.authorVeríssimo, Joana
dc.contributor.authorVikhrev, Ilya V.
dc.contributor.authorWoschitz, G.
dc.contributor.authorZając, Katarzyna
dc.contributor.authorZając, Tadeusz A.
dc.contributor.authorZanatta, David Thomas
dc.contributor.authorZieritz, Alexandra
dc.contributor.authorZogaris, Stamatis
dc.contributor.authorFroufe, Elsa
dc.date.accessioned2024-06-25T15:54:25Z
dc.date.available2024-06-25T15:54:25Z
dc.date.issued2024
dc.description.abstractThe global decline of freshwater mussels and their crucial ecological services highlight the need to understand their phylogeny, phylogeography and patterns of genetic diversity to guide conservation efforts. Such knowledge is urgently needed for Unio crassus, a highly imperilled species originally widespread throughout Europe and southwest Asia. Recent studies have resurrected several species from synonymy based on mitochondrial data, revealing U. crassus to be a complex of cryptic species. To address long-standing taxonomic uncertainties hindering effective conservation, we integrate morphometric, phylogenetic, and phylogeographic analyses to examine species diversity within the U. crassus complex across its entire range. Phylogenetic analyses were performed using cytochrome c oxidase subunit I (815 specimens from 182 populations) and, for selected specimens, whole mitogenome sequences and Anchored Hybrid Enrichment (AHE) data on ∼ 600 nuclear loci. Mito-nuclear discordance was detected, consistent with mitochondrial DNA gene flow between some species during the Pliocene and Pleistocene. Fossil-calibrated phylogenies based on AHE data support a Mediterranean origin for the U. crassus complex in the Early Miocene. The results of our integrative approach support 12 species in the group: the previously recognised Unio bruguierianus, Unio carneus, Unio crassus, Unio damascensis, Unio ionicus, Unio sesirmensis, and Unio tumidiformis, and the reinstatement of five nominal taxa: Unio desectus stat. rev., Unio gontierii stat. rev., Unio mardinensis stat. rev., Unio nanus stat. rev., and Unio vicarius stat. rev. Morphometric analyses of shell contours reveal important morphospace overlaps among these species, highlighting cryptic, but geographically structured, diversity. The distribution, taxonomy, phylogeography, and conservation of each species are succinctly described.pt_PT
dc.description.sponsorshipWe thank Ana-Maria Benedek, Monica Sîrbu and Jouni Leinikki for their assistance with the fieldwork, and to Jeroen Goud, Sankurie Pye, Fiona Ware, Emily Mitchell, and Aleksandra Skawina for their assistance with the taxonomic investigation. We would also like to thank the editor, Dr. Guillermo Ortí, and two anonymous reviewers for their time and effort in reviewing our manuscript and for their insightful comments and valuable improvements to our work. This publication is based upon work from COST Action CA18239: CONFREMU - Conservation of freshwater mussels: a pan-European approach, supported by COST (European Cooperation in Science and Technology), including STSMs, the interaction of the authors and the writing of the paper. This work was supported by the project ConBiomics: The Missing Approach for the Conservation of Freshwater Bivalves Project No. POCI-01-0145-FEDER-030286, co-financed by FEDER through POCI and by FCT - Fundaç˜ao para a Ciˆencia e a Tecnologia, through national funds. Strategic funding UIDB/04423/2020 and UIDP/04423/2020 was provided by FCT. FCT also supported DVG (2020.03848.CEECIND), EF (CEECINST/00027/ 2021/CP2789/CT0003) and MLL (2020.03608.CEECIND). INB, AVK and IVV were supported by the Russian Science Foundation under grants (19-14-00066-P), (21-17-00126) and (21-74-10130) respectively. BVB acknowledges the bioinformatics platform of UMR 8198 for the computing resources to perform time-calibrated phylogenetic analyses; this platform is in part funded by CPER research project CLIMIBIO through the French Minist`ere de l’Enseignement Sup´erieur et de la Recherche, the Agence Nationale de la Recherche, the European Fund for Regional Development (FEDER) and the region Hauts-de-France (HdF). Support to KD came from the Czech Science Foundation (19–05510S). TT and MT were supported by the National Science Fund of Bulgaria under the project ‘Conservation of freshwater mussels on the Balkan Peninsula’ (KP-06-COST-9/20.07.2022). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the United States Government.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLopes-Lima, Manuel; Geist, Juergen P.; Egg, S.; Beran, Luboš; Bikashvili, Ani; Van Bocxlaer, Bert; Bogan, Arthur E.; Bolotov, Ivan N; Chelpanovskaya, O.A.; Douda, Karel; Fernandes, Verónica; Gomes-dos-Santos, André; Gonçalves, Duarte Vasconcelos; Gürlek, Mustafa Emre; Johnson, Nathan A.; Karaouzas, Ioannis D.; Kebapçı, Ümit; Kondakov, Duarte Vasconcelos; Kuehn, Ralf; Lajtner, Jansa; Mumladze, Levan; Nagel, Karl Otto; Neubert, Eike; Österling, E. Martins; Pfeiffer, John M.; Prié, Vincent; Riccardi, Nicoletta; Sell, Jerzy; Schneider, Lea D.; Shumka, Spasse; Sîrbu, Ioan; Skujienė, Grita; Smith, Chase H.; Sousa, Ronaldo Gomes; Stöckl, K., Taskinen, Jouni k.; Teixeira, Amílcar; Todorov, Milcho T.; Trichkova, Teodora A.; Urbańska, Maria; Välilä, Santtu; Varandas, Simone Da Graça Pinto; Veríssimo, Joana; Vikhrev, Ilya V.; Woschitz, G.; Zając, Katarzyna; Zając, Tadeusz A.; Zanatta, David Thomas; Zieritz, Alexandra; Zogaris, Stamatis; Froufe, Elsa (2024). Integrative phylogenetic, phylogeographic and morphological characterisation of the Unio crassus species complex reveals cryptic diversity with important conservation implications. Molecular Phylogenetics and Evolution. ISSN 1055-7903. 195, p. 1-27pt_PT
dc.identifier.doi10.1016/j.ympev.2024.108046pt_PT
dc.identifier.issn1055-7903
dc.identifier.urihttp://hdl.handle.net/10198/29937
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcademic Presspt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationFrom freshwater to sand seas: multi-scale biogeography and conservation in North Africa
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectConservationpt_PT
dc.subjectFreshwater musselspt_PT
dc.subjectPhylogeographypt_PT
dc.subjectUnionidapt_PT
dc.titleIntegrative phylogenetic, phylogeographic and morphological characterisation of the Unio crassus species complex reveals cryptic diversity with important conservation implicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleFrom freshwater to sand seas: multi-scale biogeography and conservation in North Africa
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.03848.CEECIND%2FCP1599%2FCT0003/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.03608.CEECIND%2FCP1601%2FCP1649%2FCT0005/PT
oaire.citation.endPage27pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMolecular Phylogenetics and Evolutionpt_PT
oaire.citation.volume195pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 3ed
oaire.fundingStreamCEEC IND 3ed
person.familyNameTeixeira
person.givenNameAmilcar
person.identifier2153193
person.identifier.ciencia-id9510-3CF3-0393
person.identifier.orcid0000-0001-5336-1174
person.identifier.scopus-author-id7202385393
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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