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In vitro culture conditions of Colletotrichum gloeosporioides for spore production: a) Effect of medium composition and light on spore production

dc.contributor.authorOfferman, J.
dc.contributor.authorSousa, Maria João
dc.contributor.authorRodrigues, Paula
dc.contributor.authorMartins, Anabela
dc.contributor.authorDias, Alberto
dc.date.accessioned2008-12-22T10:59:28Z
dc.date.available2008-12-22T10:59:28Z
dc.date.issued2005
dc.description.abstractColletotrichum gloeosporioides, the imperfect stage of the ascomycete Glomerella singulata, is responsible for anthracnose in a wide variety of plant species. Hypericum perforatum, which is responsible for the synthesis of hypericin, a metabolite with important pharmaceutical applications, is one of the species sensitive to C. gloeosporioides. Since H. perforatum tolerance to C. gloeosporioides is dependent on plant variety, we intend to select tolerant varieties in vitro for metabolite production without fungicide application. C. gloeosporioides spores are needed to inoculate and induce a selective pressure on plants, but in vitro sporulation is particularly difficult to achieve. The establishment of in vitro conditions for sporulation was the main goal of the trials here presented. Two variables were tested for C. gloeosporioides spore production: medium composition and light. The effect of medium composition was tested by growing the fungus in PDA (Potato Dextrose Agar) and MMN (modified Melin & Norkrans). We also tested the influence of moving the fungus from PDA to MMN and vice-versa. Growth and spore formation was recorded for a period of three months. Light influence was tested by submitting the fungus to light and dark conditions, for both media. Growth and spore formation under these conditions was recorded for a period of two months. Preliminary results showed PDA to be a better medium for spore production in C. gloeosporioides. The presence or absence of light did not influence spore production.en
dc.description.sponsorshipPOCTI - AGR 40283-2001en
dc.identifier.citationBSPP Presidential Meeting. Nottingham, 2005en
dc.identifier.urihttp://hdl.handle.net/10198/950
dc.language.isoengen
dc.publisherThe British Society for Plant Pathologyen
dc.subjectColletotrichumen
dc.subjectSpore productionen
dc.titleIn vitro culture conditions of Colletotrichum gloeosporioides for spore production: a) Effect of medium composition and light on spore productionen
dc.typeconference object
dspace.entity.typePublication
person.familyNameSousa
person.familyNameRodrigues
person.familyNameMartins
person.givenNameMaria João
person.givenNamePaula
person.givenNameAnabela
person.identifier.ciencia-idD91E-3571-3979
person.identifier.ciencia-id2312-18AE-DA42
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.orcid0000-0002-9946-4926
person.identifier.orcid0000-0002-3789-2730
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id55230282700
person.identifier.scopus-author-id7102639646
person.identifier.scopus-author-id7203013518
rcaap.rightsopenAccessen
rcaap.typeconferenceObjecten
relation.isAuthorOfPublicationda5c7615-9844-4fdf-b4d4-64d0c8e72de0
relation.isAuthorOfPublicationb864b8c1-d66a-419c-b1ac-e6193f93eca6
relation.isAuthorOfPublication383370dd-10e5-40e6-9a15-f2201fe95581
relation.isAuthorOfPublication.latestForDiscoveryb864b8c1-d66a-419c-b1ac-e6193f93eca6

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