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Exploiting the mitogenomes of apis mellifera subspecies to develop an authentication tool to verify the entomological origin of mediterranean honeys

2024Conference object Open access
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dc.contributor.authorHonrado, Mónica
dc.contributor.authorHenriques, Dora
dc.contributor.authorSantos, Joana
dc.contributor.authorYadró Garcia, Carlos A.
dc.contributor.authorMartín-Hernández, Raquel
dc.contributor.authorNanetti, Antonio
dc.contributor.authorGonzález, Amelia Virginia
dc.contributor.authorAl Shagour, Banan
dc.contributor.authorHosri, Chadi
dc.contributor.authorFarrugia, Dylan
dc.contributor.authorGiovanni, Cilia
dc.contributor.authorZammit Mangion, Marion
dc.contributor.authorMuz, Mustafa Necati
dc.contributor.authorHaddad, Nizar
dc.contributor.authorGalea, Thomas
dc.contributor.authorHaider, Yamina
dc.contributor.authorObeidat, Wisam
dc.contributor.authorAglagane, Abdessamad
dc.contributor.authorArab, Alireza
dc.contributor.authorVarnava, Andri
dc.contributor.authorEissa, Asmaa Anwar
dc.contributor.authorMuz, Dilek
dc.contributor.authorHatjina, Fani
dc.contributor.authorLamghari, Fouad
dc.contributor.authorArruda, James
dc.contributor.authorCaristos Caristos, Leonidas
dc.contributor.authorPinto, M. Alice
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2024-10-08T09:46:32Z
dc.date.available2024-10-08T09:46:32Z
dc.date.issued2024
dc.description.abstractHoney is highly susceptible to adulteration. Currently, the assessment of its geographical origin remains one of the most difficult tasks, which is typically performed by melyssopalynology. Recently, the attention has shifted towards indirect approaches such as the entomological origin based on geographical distribution patterns of honey bee subspecies. Although queens’ trade has impacted the natural subspecies distribution, honeys produced with autochthonous bees or bearing a Protected Designation of Origin specifying the producing honey bee subspecies, offer a unique avenue for authentication. In the MEDIBEES project, we aim to develop a DNA-metabarcoding approach to authenticate honey's entomological origin focusing on mitochondrial lineages A, M, C, and O. To achieve this goal, the DNA from 1251 honey bees representing 16 subspecies (A.m. sahariensis, A.m. intermissa, A.m. siciliana, A.m. ruttneri, A.m. iberiensis, A.m. ligustica, A.m. macedonica, A.m. adami, A.m. cecropia, A.m. cypria, A.m. caucasia, A.m. meda, A.m. anatoliaca, A.m. syriaca, A.m. jemenitica, A.m. lamarcki) was extracted and the whole genome sequenced. From those, 740 mitogenomes were assembled using the MitoZ software. The quality of the assembled mitogenome was assessed by aligning all the sequences using MEGA and 348 samples were deleted. Finally, a phylogenetic analysis was conducted to eliminate non-local subspecies, resulting in a total of 326 mitogenomes. This dataset was used for calculating the fixation index (FST) pairwise values, and a sliding window of 400bp was used to identify single nucleotide polymorphisms that effectively differentiate (FST>0.98) the four lineages, enabling the identification of promising regions for primer design. In this study, three regions were identified that discriminate the four maternal lineages while showing an appropriate length for metabarcoding, namely in the COI, ND1 gene, and CYTB genes.pt_PT
dc.description.sponsorshipThis work was funded by PRIMA project “MEDIBEES” and national funds through FCT/MCTES (PIDDAC): UIDB/00690/2020|UIDP/00690/2020, LA/P/0007/2020. M.Honrado acknowledges the PhD scholarship (2021.08119.BD).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHonrado, Mónica; Henriques, Dora; Santos, Joana; Yadró Garcia, Carlos A.; Martín-Hernández, Raquel; Nanetti, Antonio; González, Amelia Virginia; Al Shagour, Banan; Hosri, Chadi;Farrugia, Dylan; Giovanni, Cilia; Zammit Mangion, Marion; Muz, Mustafa Necati; Haddad, Nizar; Galea, Thomas; Haider, Yamina; Obeidat, Wisam; Aglagane, Abdessamad; Arab, Alireza; Varnava, Andri; Eissa, Asmaa Anwar; Muz, Dilek; Hatjina, Fani; Lamghari, Fouad; Arruda, James; Caristos Caristos, Leonidas; Pinto, M. Alice; Amaral, Joana S. (2024). Exploiting the mitogenomes of apis mellifera subspecies to develop an authentication tool to verify the entomological origin of mediterranean honeys. In 10th congress of Apidology. Tallinpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/30350
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherEstonian University of Life Sciencespt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMitogenomespt_PT
dc.subjectHoneypt_PT
dc.subjectEntomological originpt_PT
dc.subjectMediterraneanpt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleExploiting the mitogenomes of apis mellifera subspecies to develop an authentication tool to verify the entomological origin of mediterranean honeyspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.08119.BD/PT
oaire.citation.conferencePlaceTallinpt_PT
oaire.citation.endPage163pt_PT
oaire.citation.startPage162pt_PT
oaire.citation.title10th Congress of Apidologypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameHonrado
person.familyNameHenriques
person.familyNameYadró García
person.familyNamePinto
person.familyNameAmaral
person.givenNameMónica
person.givenNameDora
person.givenNameCarlos A.
person.givenNameM. Alice
person.givenNameJoana S.
person.identifier.ciencia-id4712-B40B-4B0E
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-5126-4693
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0002-3648-7303
person.identifier.scopus-author-id55761737300
person.identifier.scopus-author-id8085507800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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