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Characterization of phenolic and polysaccharidic extracts of Ganoderma lucidum by chromatographic techniques

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dc.contributor.authorHeleno, Sandrina A.
dc.contributor.authorBarros, Lillian
dc.contributor.authorMartins, Anabela
dc.contributor.authorQueiroz, Maria João R.P.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2012-08-17T13:31:26Z
dc.date.available2012-08-17T13:31:26Z
dc.date.issued2012
dc.description.abstractGanoderma lucidum is one of the most extensively studied mushrooms due to its medicinal properties such as the capacity to lower the risk of cancer, liver, and heart diseases and to boost the immune system [1]. The beneficial health properties of Ganoderma are attributed to a wide variety of bioactive components present in the fruiting body, mycelium, and spores [2]. Herein, a systematic study was carried out in order to compare the antioxidant activity of phenolic and polysaccharidic extracts from fruiting body, spores and mycelium, obtained in three different culture media, of G. lucidum from Northeast Portugal. Phenolic extracts were characterized by high-performance liquid chromatography coupled to photodiode array detection, while polysaccharidic extracts were hydrolysed and further characterized by HPLC and refraction index detection. The bioactive compounds were related to free radical scavenging properties, reducing power and lipid peroxidation inhibition. The phenolic extracts (Ph) of fruiting body (FB) and mycelia (M) proved to have higher antioxidant potential than their corresponding polysaccharidic extracts (Ps). For the spores (S), the opposite was observed. FB-Ph was the most potent (EC50 values ≤ 0.62 mg/ml) and revealed the highest content in total phenolics (28.64 mg GAE/g extract) and phenolic acids such as p-hydroxybenzoic and p-coumaric acids. Among the polysaccharidic extracts, S-Ps gave the best antioxidant activity (EC50 values ≤ 2.02 mg/ml), but the highest total polysaccharides (14.50 and 13.98 mg PE/g extract for M-PDA-Ps and M-sMMN-Ps, respectively) and individual sugars were observed in mycelia obtained from solid culture media. The free radical scavenging properties, reducing power and lipid peroxidation inhibition of G. lucidum are more correlated with phenolic compounds than with polysaccharides.por
dc.identifier.citationHeleno, Sandrina A.; Barros, Lillian; Martins, Anabela; Queiroz, Maria João R.P.; Ferreira, Isabel C.F.R. (2012). Characterization of phenolic and polysaccharidic extracts of Ganoderma lucidum by chromatographic techniques. In 7º Encontro Nacional de Cromatografia. Portopor
dc.identifier.urihttp://hdl.handle.net/10198/7353
dc.language.isoengpor
dc.peerreviewedyespor
dc.relationBPD/4609/2008
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationIMPORTANT HUMAN METABOLITES OF PHENOLIC ACIDS FROM DIET WITH WILD EDIBLE MUSHROOMS: CHEMICAL SYNTHESIS AND STUDIES OF THEIR ANTIOXIDANT AND ANTITUMOR PROPERTIES
dc.titleCharacterization of phenolic and polysaccharidic extracts of Ganoderma lucidum by chromatographic techniquespor
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitleIMPORTANT HUMAN METABOLITES OF PHENOLIC ACIDS FROM DIET WITH WILD EDIBLE MUSHROOMS: CHEMICAL SYNTHESIS AND STUDIES OF THEIR ANTIOXIDANT AND ANTITUMOR PROPERTIES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70304%2F2010/PT
oaire.citation.conferencePlacePortopor
oaire.citation.title7º Encontro Nacional de Cromatografia, 9 a 11 de Janeiro de 2012por
oaire.fundingStream5876-PPCDTI
person.familyNameHeleno
person.familyNameBarros
person.familyNameMartins
person.familyNameFerreira
person.givenNameSandrina A.
person.givenNameLillian
person.givenNameAnabela
person.givenNameIsabel C.F.R.
person.identifier469085
person.identifier144781
person.identifier.ciencia-id8B18-3095-6FC9
person.identifier.ciencia-id9616-35CB-D001
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.ciencia-id9418-CF95-9919
person.identifier.orcid0000-0001-7224-1098
person.identifier.orcid0000-0002-9050-5189
person.identifier.orcid0000-0001-6218-4413
person.identifier.orcid0000-0003-4910-4882
person.identifier.ridL-5951-2014
person.identifier.ridJ-3600-2013
person.identifier.ridG-5488-2013
person.identifier.ridE-8500-2013
person.identifier.scopus-author-id30067731800
person.identifier.scopus-author-id35236343600
person.identifier.scopus-author-id7203013518
person.identifier.scopus-author-id36868826600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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