Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products

Amaral, Joana S.; Soares, Sónia; Mafra, Isabel; Oliveira, Beatriz

Publication

Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products

2009Conference paper

dc.contributor.author	Amaral, Joana S.
dc.contributor.author	Soares, Sónia
dc.contributor.author	Mafra, Isabel
dc.contributor.author	Oliveira, Beatriz
dc.date.accessioned	2011-06-01T13:24:09Z
dc.date.available	2011-06-01T13:24:09Z
dc.date.issued	2009
dc.description.abstract	In this work, a polymerase chain reaction (PCR) technique was used for soybean detection in processed meat products. The methodology was optimized using reference binary samples with different known percentages of pork meat and soybean protein, prepared in the laboratory. To evaluate the effect of thermal treatment, binary mixtures were submitted to thermal treatment at 121ºC for 5 min. The species-specific PCR targeting the lectin gene allowed the detection limit of 0.01 ng of soybean DNA in raw materials. The PCR method was successfully applied on 18 commercial Frankfurt sausages.	por
dc.identifier.citation	Amaral, J.S.; Soares, S.; Mafra, I.; Oliveira, M.B.P.P (2009). Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products. In Actas do 9º Encontro de Química dos Alimentos. Angra do Heroísmo. CD-ROM.	por
dc.identifier.uri	http://hdl.handle.net/10198/4866
dc.language.iso	por	por
dc.peerreviewed	yes	por
dc.subject	Soybean	por
dc.subject	Allergens	por
dc.subject	Meat products	por
dc.subject	PCR	por
dc.title	Polymerase chain reaction for the detection of allergens: the case of soybean in processed meat products	por
dc.type	conference paper
dspace.entity.type	Publication
oaire.citation.conferencePlace	Angra do Heroísmo	por
oaire.citation.title	Actas do 9º Encontro de Química dos Alimentos, CD-ROM	por
person.familyName	Amaral
person.givenName	Joana S.
person.identifier.ciencia-id	5319-7DE8-BEDA
person.identifier.orcid	0000-0002-3648-7303
rcaap.rights	restrictedAccess	por
rcaap.type	conferenceObject	por
relation.isAuthorOfPublication	42be2cf4-adc4-4e7f-ac60-7aab515b38cd
relation.isAuthorOfPublication.latestForDiscovery	42be2cf4-adc4-4e7f-ac60-7aab515b38cd