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Honeybee gut microbiota as an emerging endpoint for pesticide risk assessments

2024Conference object Open access
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dc.contributor.authorRosa-Fontana, Annelise
dc.contributor.authorAguado-López, Daniel
dc.contributor.authorJabal-Uriel, Clara
dc.contributor.authorMartín-Hernández, Raquel
dc.contributor.authorHiges, Mariano
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.contributor.authorTosi, Simone
dc.contributor.authorRodríguez Gomez, Juan Miguel
dc.date.accessioned2024-10-04T07:54:37Z
dc.date.available2024-10-04T07:54:37Z
dc.date.issued2024
dc.description.abstractA recent roadmap for the integration of environmental microbiotas in risk assessments under the European Food Safety Authority (EFSA) remit has been published. Healthy honeybee gut microbiota has emerged as a promising avenue to protect bees against stressors. Honeybees exhibit a consistent core microbiota, and dysbiosis, as part of a multiple stressor system, may be an indicator of adverse scenarios. We therefore investigated the honeybee gut microbiota of Apis mellifera carnica workers exposed to a single concentration of the insecticide flupyradifurone (FPF, 36ppm). The laboratory trials were carried out in accordance with official protocols (OECD Nº 245). The abdomen of each bee was separated from the thorax, and DNA extraction was performed individually. Full-length 16s rRNA amplicon metagenomic was sequenced through PacBio sequel II (HiFi/CCS mode). The absolute abundance of four bacterial genera constituting the core honeybee microbiota unveiled a Lactobacillus-dominated gut in both treated and non-treated bees. Treated bees exhibited a twofold increase in the bacterial load of Snodgrassella, contrasting with a 50% reduction in the Bifidobacterium load and the complete absence of Gilliamella as compared to the untreated bees. Our findings revealed that FPF disrupted the honeybee gut microbiota. We have developed a new approach, overlooked in risk assessments studies so far, to assess the impact of pesticides bee health until now. Thus, we propose its use as a novel endpoint in pesticide risk assessments. Current risk assessments are performed in a tiered approach, i. e., moving from laboratory assays (first screening) to semi field and field studies, and require no sublethal effect assessments. We therefore advocate for the inclusion of honeybee gut microbiota dysbiosis as a sublethal effect in the first screening step of risk assessments, and as a key parameter to assess pollinator’s health.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRosa-Fontana, Annelise; Aguado-López, Daniel; Jabal-Uriel, Clara; Martín-Hernández, Raquel; Higes, Mariano; Pinto, M. Alice; Henriques, Dora; Tosi, Simone; Rodríguez Gomez, Juan Miguel (2024). Honeybee gut microbiota as an emerging endpoint for pesticide risk assessments. In 10th congress of Apidology. Tallinpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/30317
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherEurbee10pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHoney bee microbiotapt_PT
dc.subjectPesticide risk assessementspt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleHoneybee gut microbiota as an emerging endpoint for pesticide risk assessmentspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceTallinpt_PT
oaire.citation.endPage176pt_PT
oaire.citation.startPage176pt_PT
oaire.citation.title10th Congress of Apidologypt_PT
person.familyNamePinto
person.familyNameHenriques
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublicationd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isAuthorOfPublication.latestForDiscoveryd2abd09f-a90c-4cfb-9a60-7fc32f56184d

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