Logo do repositório
 
Publicação

Comparative Analysis of DNA Extraction Methods for Individual Varroa destructor

2024Documento de conferência Acesso aberto
Ver registo simples
dc.contributor.authorCosta, Maíra
dc.contributor.authorLopes, Ana
dc.contributor.authorYadró Garcia, Carlos A.
dc.contributor.authorVitrio, Nathalia
dc.contributor.authorGonçalves, Telma
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.date.accessioned2024-10-02T08:59:30Z
dc.date.available2024-10-02T08:59:30Z
dc.date.issued2024
dc.description.abstractThe ectoparasitic mite Varroa destructor is one of the major honey bee threats and it is associated to population worldwide decline. Genetic analyses using the mtDNA of V. destructor are fundamental for establishing the taxonomy and distribution of the mites. Consequently, low-quality DNA can lead to inaccurate or inconsistent data, making genetic interpretation more challenging. In this study, we compared the concentration and quality of DNA extracted from individual female V. destructor using two different commercial kits, aiming to identify the optimal method for obtaining high-quality DNA. Total DNA was extracted from mites using both an automated and manual extracted method. In addition, manual kit extraction tested three incubation procedure (1h, 5h, and overnight). DNA concentration was quantified using three different instruments: the SpectroStarVR Nano LVis Plate spectrophotometer, the Quantus™ Fluorometer apparatus, and NanoDrop™. The manual extraction DNA concentration did not vary across incubation times and the concentration values varied between 0.240-0.545 ng/μl (Quantus), 0.72-4.49 ng/μl (spectrophotometer), and 0.0-1.47 ng/μl (NanoDrop). While extraction automatic approach yielded higher respectively 0.483-0.631 ng/μl, 20.33-9.0 ng/μl, and 5.3-6.8 ng/μl. In conclusion, the automated kit extraction seems to be the best extraction method since it produced the higher-concentration DNA using only one individual mite.pt_PT
dc.description.sponsorshipMITE- Varroa e vírus transmitidos: Monitorização de mutações e desenvolvimento de ferramentas moleculares inovadoras” com financiamento do programa apícola nacional 2023-2027.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, Maíra; Lopes, Ana; Yadró Garcia, Carlos A.; Vitrio, Nathalia; Gonçalves, Telma; Pinto, M. Alice; Henriques, Dora (2024). Comparative Analysis of DNA Extraction Methods for Individual Varroa destructor. In Third International Congress on Bee Sciences. Online. ISBN 978-605-71368-8-6pt_PT
dc.identifier.isbn978-605-71368-8-6
dc.identifier.urihttp://hdl.handle.net/10198/30304
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUlas Acarözpt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDNA extractionpt_PT
dc.subjectDNA quantificationpt_PT
dc.subjectDNA qualitypt_PT
dc.subjectVarroa destructorpt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleComparative Analysis of DNA Extraction Methods for Individual Varroa destructorpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumberUIDB/00690/2020
oaire.awardNumberUIDP/00690/2020
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.conferencePlaceOnlinept_PT
oaire.citation.endPage134pt_PT
oaire.citation.startPage134pt_PT
oaire.citation.titleThird International congress on Bee Sciencespt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCosta
person.familyNameLopes
person.familyNameYadró Garcia
person.familyNameGonçalves
person.familyNamePinto
person.familyNameHenriques
person.givenNameMaíra
person.givenNameAna Rita
person.givenNameCarlos A.
person.givenNameTelma
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-id6A19-43EC-92E5
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0001-6697-6581
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0009-0001-9346-7384
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationc7b97b29-3139-43ff-ae85-23f613e280ed
relation.isAuthorOfPublication5c8dc714-e63c-4ac6-882e-92deafa4bbe8
relation.isAuthorOfPublication59212a6c-fc6a-45fd-b37a-51e90926c9e3
relation.isAuthorOfPublicatione27e63e0-e773-41e4-81a5-63d104a6040a
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublicationd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isAuthorOfPublication.latestForDiscoveryd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication0aac8939-28c2-46f4-ab6b-439dba7f9942
relation.isProjectOfPublication.latestForDiscovery0aac8939-28c2-46f4-ab6b-439dba7f9942

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
icbees2.pdf
Tamanho:
720.17 KB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.75 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIMO - Resumos em Proceedings Não Indexados à WoS/Scopus