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Advisor(s)
Abstract(s)
Sugar profiles of different almond and chestnut
cultivars were obtained by high-performance liquid chromatography
(HPLC), by means of a refractive index (RI)
detector. A solid-liquid extraction procedure was used in
defatted and dried samples. The chromatographic separation
was achieved using a Eurospher 100-5 NH2 column using an
isocratic elution with acetonitrile/water (70:30, v/v) at a flow
rate of 1.0 ml/min. All the compounds were separated in
16 min. The method was optimized and proved to be
reproducible and accurate. Generally, more than 95% of
sugars were identified for both matrixes. Sugars profiles
were quite homogeneous for almond cultivars; sucrose was
the main sugar (11.46±0.14 in Marcona to 22.23±0.59 in
Ferragnes g/100 g of dried weight), followed by raffinose
(0.71±0.05 in Ferraduel to 2.11±0.29 in Duro Italiano),
glucose (0.42±0.12 in Pegarinhos two seeded to 1.47±0.19
in Ferragnes) and fructose (0.11±0.02 in Pegarinhos two
seeded to 0.59±0.05 in Gloriette). Commercial cultivars
proved to have higher sucrose contents, except in the case
of Marcona. Nevertheless, chestnut cultivars revealed a
high heterogeneity. Sucrose was the main sugar in Aveleira
(22.05±1.48), Judia (23.30±0.83) and Longal (9.56± 0.91), while glucose was slightly prevalent in Boa Ventura
(6.63±0.49). The observed variance could serve for intercultivar
discrimination.
Description
Keywords
Castanea sativa Mill. HPLC-RI Prunus dulcis Sugars profiles
Citation
Barreira, J.C.M.; Pereira, J. A.; Oliveira, M.B.P.P.; Ferreira, I.C.F.R. (2010). Sugar profiles of different chestnut (Castanea sativa Mill.) and almond (Prunus dulcis L.) cultivars by HPLC-RI. Plant Foods for Human Nutrition (Formerly Qualitas Plantarum). ISSN 0921-9668. 65:1, p. 38-43
Publisher
Springer