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Glycyrrhiza glabra hydroethanolic extract and manuka honey alone and in combination inhibit bacterial and fungal planktonic cells and biofilms

2024Journal article Open access
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dc.contributor.authorGomes, Fernanda
dc.contributor.authorDias, Maria Inês
dc.contributor.authorRodrigues, Maria Elisa
dc.contributor.authorBarros, Lillian
dc.contributor.authorHenriques, Mariana
dc.date.accessioned2024-06-27T13:37:43Z
dc.date.available2024-06-27T13:37:43Z
dc.date.issued2024
dc.description.abstractThe natural tendency of polymicrobial infections and co-habitation of bacteria and Candida are currently well-recognized and reported as highly fatal. This kind of interaction is usually associated with drug tolerance, infection relapse, persistence and high mortality. The interest in natural products has emerged and their potential applicability as therapeutic strategies is being constantly explored. Purpose: The main goal of this work is to evaluate the effect of the hydroethanolic extract of Glycyrrhiza glabra, and Manuka honey, in Staphylococcus aureus and Candida tropicalis planktonic cells and single and mixed biofilms. Methods: The effect of both the G. glabra extract and the honey was evaluated by determination of the minimum inhibitory/bactericidal/fungicidal concentrations and by determination of their antibiofilm activity (alone and in combination) on single and mixed-species biofilms. In addition, phenolic compounds were characterized in the hydroethanolic extract with high performance liquid chromatography and mass spectrometry. Results: Among the phenolic compounds identified in the G. glabra extract, flavonoids were the most abundant (≈75 % of the total phenolic compounds), with liquiritigenin being the most representative. S. aureus and C. tropicalis were susceptible to both antimicrobials acting alone. When tested in combination, a significative synergism was observed against C. tropicalis single biofilm formation. Instead, the combination showed an additive effect against mixed biofilms of S. aureus and C. tropicalis. In general the combination G. glabra+honey showed a broader antibiofilm effect than each partner alone. Conclusion: The results showed the good anti S. aureus and C. tropicalis effect of G. glabra and manuka honey against both pathogens tested and highlighted their potential use in combination as therapeutic strategy to combat S. aureus, C. tropicalis and mixed species infections particularly when growing as biofilms.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/ MCTES under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020; as also for CIMO UIDB/00690/2020 (DOI: 10.54499/UIDB/00690/2020) and UIDP/00690/2020 (DOI: 10.54499/UIDP/00690/2020); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020); national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros and M.I. Dias contracts.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGomes, Fernanda; Dias, Maria Inês; Rodrigues, Maria Elisa; Barros, Lillian; Henriques, Mariana (2024). Glycyrrhiza glabra hydroethanolic extract and manuka honey alone and in combination inhibit bacterial and fungal planktonic cells and biofilms. Phytomedicine Plus. ISSN 2667-0313. 4:2, p. 1-8pt_PT
dc.identifier.doi10.1016/j.phyplu.2024.100561pt_PT
dc.identifier.issn2667-0313
dc.identifier.urihttp://hdl.handle.net/10198/29958
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relationAssociate Laboratory on Biotechnology, Bioengineering and microELectromechanical Systems
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.relationAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectGlycyrrhiza glabrapt_PT
dc.subjectHoneypt_PT
dc.subjectPhenolic profilept_PT
dc.titleGlycyrrhiza glabra hydroethanolic extract and manuka honey alone and in combination inhibit bacterial and fungal planktonic cells and biofilmspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardTitleAssociate Laboratory on Biotechnology, Bioengineering and microELectromechanical Systems
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardTitleAssociate Laboratory for Sustainability and Tecnology in Mountain Regions
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0029%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT
oaire.citation.endPage8pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePhytomedicine Pluspt_PT
oaire.citation.volume4pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameDias
person.familyNameBarros
person.givenNameMaria Inês
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-id2A13-4BE6-C7CF
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-8744-7814
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-8242-2013
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id54388787000
person.identifier.scopus-author-id35236343600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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