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Development of a new approach based on NADH dehydrogenase subunit 1(ND1) marker and high resolution melting (HRM) analysis towards the authentication of the geographical origin of honey

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dc.contributor.authorHonrado, Mónica
dc.contributor.authorPinto, M. Alice
dc.contributor.authorLopes, Ana
dc.contributor.authorHenriques, Dora
dc.contributor.authorAmaral, Joana S.
dc.date.accessioned2021-07-28T09:39:33Z
dc.date.available2021-07-28T09:39:33Z
dc.date.issued2021
dc.description.abstractIn the present study, an approach for verifying the geographical origin of honey based on its entomological origin is proposed. The method was developed based on the polymerase chain reaction (PCR) amplification of a new markercontaining different single nucleotide polymorphisms characteristic of honeybees of different mitochondrial (mtDNA) lineages, therefore generating different fluorescent curves on HRM analysis. The method was successfully applied to honeys from Portugal and Italy, expected to be from lineages A and C, respectively, demonstrating their origin compliance.pt_PT
dc.description.sponsorshipThe authors are grateful to Programa Nacional Apícola 2020-2022 for funding the project “AUTENT+ Desenvolvimento de abordagens inovadoras com vista à valorização e exploração do potencial de mercado do mel Português”, to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO UIDB/00690/2020, to Fenapícola and Capemel for supplying the Portuguese honeys and to Dr. Antonio Nanetti (CREA-AA) for the Italian honeys. D. Henriques is supported by the project Bee Happy (POCI-01-0145-FEDER-029871) funded by FEDER, COMPETE 2020-POCIand FCT and A.R. Lopes by the PhD scholarship funded by the FCT SFRH/BD/143627/2019.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHonrado, Mónica; Pinto, M. Alice; Lopes, Ana Rita; Henriques, Dora; Amaral, Joana S. (2021). Development of a new approach based on NADH dehydrogenase subunit 1(ND1) marker and high resolution melting (HRM) analysis towards the authentication of the geographical origin of honey. In Advances in Food Chemistry 2021 Conference (AdFoodChem 2021). Onlinept_PT
dc.identifier.urihttp://hdl.handle.net/10198/23785
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHoney authenticitypt_PT
dc.subjectApis mellifera subspeciespt_PT
dc.subjectHigh resolution melting (HRM)pt_PT
dc.titleDevelopment of a new approach based on NADH dehydrogenase subunit 1(ND1) marker and high resolution melting (HRM) analysis towards the authentication of the geographical origin of honeypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceon-linept_PT
oaire.citation.endPage73pt_PT
oaire.citation.startPage72pt_PT
oaire.citation.titleAdvances in Food Chemistry International Conferencept_PT
person.familyNameHonrado
person.familyNamePinto
person.familyNameLopes
person.familyNameHenriques
person.familyNameAmaral
person.givenNameMónica
person.givenNameM. Alice
person.givenNameAna
person.givenNameDora
person.givenNameJoana S.
person.identifier.ciencia-id4712-B40B-4B0E
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-id291F-986F-07DA
person.identifier.ciencia-id5319-7DE8-BEDA
person.identifier.orcid0000-0002-5126-4693
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0001-7530-682X
person.identifier.orcid0000-0002-3648-7303
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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