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Advisor(s)
Abstract(s)
In the present study, an approach for verifying the geographical origin of honey based on its entomological origin is proposed. The method was developed based on the polymerase chain reaction (PCR) amplification of a new markercontaining different single nucleotide polymorphisms characteristic of honeybees of different mitochondrial (mtDNA) lineages, therefore generating different fluorescent curves on HRM analysis. The method was successfully applied to honeys from Portugal and Italy, expected to be from lineages A and C, respectively, demonstrating their origin compliance.
Description
Keywords
Honey authenticity Apis mellifera subspecies High resolution melting (HRM)
Citation
Honrado, Mónica; Pinto, M. Alice; Lopes, Ana Rita; Henriques, Dora; Amaral, Joana S. (2021). Development of a new approach based on NADH dehydrogenase subunit 1(ND1) marker and high resolution melting (HRM) analysis towards the authentication of the geographical origin of honey. In Advances in Food Chemistry 2021 Conference (AdFoodChem 2021). Online