Publication
Honey bee collected pollen for botanical identification via its2 metabarcoding: a comparison of preservation methods for citizen science
| dc.contributor.author | Quaresma, Andreia | |
| dc.contributor.author | Brodschneider, Robert | |
| dc.contributor.author | Gratzer, Kristina | |
| dc.contributor.author | Gray, Alison | |
| dc.contributor.author | Keller, Alexander | |
| dc.contributor.author | Kilpinen, Ole | |
| dc.contributor.author | Rufino, José | |
| dc.contributor.author | Steen, Jozef van der | |
| dc.contributor.author | Vejsnaes, Flemming | |
| dc.contributor.author | Pinto, M. Alice | |
| dc.date.accessioned | 2023-05-26T13:56:13Z | |
| dc.date.available | 2023-05-26T13:56:13Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | DNA metabarcoding is emerging as a powerful method for botanical identification of bee-collected pollen, allowing analysis of hundreds of samples in a single high-throughput sequencing run, therefore offering unprecedented scale in citizen science projects. Biases in metabarcoding can be introduced at any stage of sample processing and preservation is the first step of the pipeline. Hence, it is important to test whether the pollen preservation method influences metabarcoding performance. While in metabarcoding studies pollen has typically been preserved at −20°C, this is not the best method to be applied by citizen scientists. Here, we compared the freezing method (FRZ) with ethanol (EtOH), silica gel (SG) and room temperature (RT) in 87 pollen samples collected from hives in Austria and Denmark. | pt_PT |
| dc.description.sponsorship | AQ acknowledges the PhD scholarship (DFA/BD/5155/2020) funded by FCT. This study was funded by INSIGNIA “Environmental monitoring of pesticides use through honey bees” (SANTE/E4/SI2.788418-SI2.788452). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Quaresma, Andreia; Brodschneider, Robert; Gratzer, Kristina; Gray, Alison; Keller, Alexander; Kilpinen, Ole; Rufino, José; Van der Steen, Jozef; Vejsnaes, Flemming; Pinto, M. Alice (2022). Honey bee collected pollen for botanical identification via its2 metabarcoding: a comparison of preservation methods for citizen science. In EurBee 9 - 9th European Congress of Apidology. Belgrade | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10198/28381 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | University of Belgrade | pt_PT |
| dc.relation | DFA/BD/5155/2020 | pt_PT |
| dc.relation | SANTE/E4/SI2.788418- SI2.788452 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Pollen DNA metabarcoding | pt_PT |
| dc.subject | Preservation bias | pt_PT |
| dc.subject | Citizen science | pt_PT |
| dc.title | Honey bee collected pollen for botanical identification via its2 metabarcoding: a comparison of preservation methods for citizen science | pt_PT |
| dc.type | conference poster | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Belgrade | pt_PT |
| oaire.citation.title | EurBee 9 - 9th European Congress of Apidology | pt_PT |
| person.familyName | Quaresma | |
| person.familyName | Rufino | |
| person.familyName | Pinto | |
| person.givenName | Andreia | |
| person.givenName | José | |
| person.givenName | M. Alice | |
| person.identifier.ciencia-id | 4F1A-4E4A-3F23 | |
| person.identifier.ciencia-id | C414-F47F-6323 | |
| person.identifier.ciencia-id | F814-A1D0-8318 | |
| person.identifier.orcid | 0000-0002-8678-5800 | |
| person.identifier.orcid | 0000-0002-1344-8264 | |
| person.identifier.orcid | 0000-0001-9663-8399 | |
| person.identifier.scopus-author-id | 57119742600 | |
| person.identifier.scopus-author-id | 55947199100 | |
| person.identifier.scopus-author-id | 8085507800 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
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