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Phenolic profile, antioxidant and antibacterial properties of Juglans regia L. (walnut) leaves from the Northeast of Portugal

2019Journal article Open access
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dc.contributor.authorVieira, Vanessa
dc.contributor.authorPereira, Carla
dc.contributor.authorPires, Tânia C.S.P.
dc.contributor.authorCalhelha, Ricardo C.
dc.contributor.authorAlves, Maria José
dc.contributor.authorFerreira, Olga
dc.contributor.authorBarros, Lillian
dc.contributor.authorFerreira, Isabel C.F.R.
dc.date.accessioned2019-04-30T11:03:52Z
dc.date.available2019-04-30T11:03:52Z
dc.date.issued2019
dc.description.abstractJuglans regia L. (walnut tree) is a recognized source of bioactive compounds with potential health benefits. In this work, hydroethanolic extracts of J. regia leaves were obtained by heat assisted extraction from different Portuguese samples in two phenological stages (green and yellow leaves) aiming to assess the impact of seasonal variations. The samples were compared regarding their phenolic composition and bioactivity. Seventeen phenolic compounds were identified by liquid chromatography combined with a diode array detector and electrospray ionization mass spectrometer (LC-DAD-ESI/MS n ): six phenolic acids, ten flavonoids and one tetralone derivative. The green leaves extracts presented a higher amount of total phenolic compounds (29.70 ± 0.03 mg/g extract) compared with the yellow leaves (23.26 ± 0.06 mg/g extract). In particular, yellow samples were richer in flavonoids (17.4 ± 0.2 mg/g extract; mainly quercetin-3-O-glucoside: 3.64 ± 0.01 mg/g extract), while the green ones presented higher phenolic acids content (16.7 ± 0.2 mg/g extract; mainly trans 3-p-coumaroylquinic acid: 6.9 ± 0.5 mg/g extract). Green leaves extract also presented higher antioxidant potential, achieving IC 50 values around 32 ± 2 μg/mL and 26.8 ± 0.2 μg/mL for the oxidative haemolysis inhibition and the thiobarbituric acid reactive substances assays, respectively. Furthermore, only green leaves samples showed anti-inflammatory potential. The cytotoxic evaluations revealed similar anti-proliferative action of both extracts against the tumor cell lines tested. Also, an analogous anti-bacterial potential of the extracts was observed, with preferential action against Gram-positive clinical isolated bacteria, with lower minimum inhibitory concentration (MIC) values for Enterococcus faecalis and Listeria monocytogenes (MIC = 2.5 mg/mL). Therefore, the present study suggests the use of walnut leaves as a source of active ingredients without hepatotoxic effects to be used in different applications in the food or pharmaceutical areaspt_PT
dc.description.sponsorshipThe authors thank the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), L. Barros, R. Calhelha and Carla Pereira contracts and V. Vieira (SFRH/BD/108487/2015) and T. Pires (SFRH/BD/129551/2017) grants. To UID/CTM/50011/2019 (CICECO), financed by national funds through the FCT/MCTES . To POCI-01-0145-FEDER-006984 (LA LSRE-LCM), funded by ERDF , through POCI-COMPETE2020 and FCT . To project AllNat - POCI-01-0145-FEDER-030463, financed by national funds through the FCT, and by COMPETE and Portugal2020, and to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E .pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVieira, Vanessa; Pereira, Carla; Pires, Tânia C.S.P.; Calhelha, Ricardo C.; Alves, Maria José; Ferreira, Olga; Barros, Lillian; Ferreira, Isabel C.F.R. (2019). Phenolic profile, antioxidant and antibacterial properties of Juglans regia L. (walnut) leaves from the Northeast of Portugal. Industrial Crops and Products. ISSN 0926-6690. 134, p. 347-355pt_PT
dc.identifier.doi10.1016/j.indcrop.2019.04.020pt_PT
dc.identifier.issn0926-6690
dc.identifier.urihttp://hdl.handle.net/10198/19287
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationUsing natural deep eutectic solvents for the extraction of bioactive compounds from plant material: valorizing walnut residues through the development of bio-based semi-solid formulas for topical use
dc.relationBio-based coloring agents for food applications: a “biorefinery” approach from the bio-waste of blueberry fruits and edible petals
dc.relationCICECO-Aveiro Institute of Materials
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnti-inflammatorypt_PT
dc.subjectAntibacterialpt_PT
dc.subjectAntioxidantspt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectJuglans regia L. leavespt_PT
dc.subjectPhenolic compoundspt_PT
dc.titlePhenolic profile, antioxidant and antibacterial properties of Juglans regia L. (walnut) leaves from the Northeast of Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUsing natural deep eutectic solvents for the extraction of bioactive compounds from plant material: valorizing walnut residues through the development of bio-based semi-solid formulas for topical use
oaire.awardTitleBio-based coloring agents for food applications: a “biorefinery” approach from the bio-waste of blueberry fruits and edible petals
oaire.awardTitleCICECO-Aveiro Institute of Materials
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00690%2F2013/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCTM%2F50011%2F2019/PT
oaire.citation.endPage355pt_PT
oaire.citation.startPage347pt_PT
oaire.citation.titleIndustrial Crops and Productspt_PT
oaire.citation.volume134pt_PT
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oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePereira
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person.givenNameTânia C.S.P.
person.givenNameRicardo C.
person.givenNameMaria José
person.givenNameOlga
person.givenNameLillian
person.givenNameIsabel C.F.R.
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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