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Advisor(s)
Abstract(s)
Protocols used for artificial insemination involve the effective synchronization of the estrous cycle by the
administration of exogenous hormones including progesterone analogs. Although widespread in the world, the
current techniques present many disadvantages that need to be overcome such as the time dependency of progesterone
levels in the blood and the need to remove the release dispositive. Nanotechnology may offer the tools
for it, and solid lipid nanoparticles (SLN) are a promising candidate to be used as nanocarriers for progesterone.
In this work, progesterone-loaded SLNs were investigated in the regulation of the estrous cycle in female rats.
First, the obtention of the SLN was investigated to determine how experimental parameters may affect the
encapsulation efficiency of progesterone and the morphology of the nanoparticles. Also, in vitro release profiles of
progesterone were also evaluated. Sexual receptivity, mutagenicity, hepatic inflammation and steatosis, hormone
measurement, and biometric parameters of pups were also determined. Progesterone-loaded SLN induced estrous
cycle synchronization and were able to maintain the estrous phase of the estrous cycle while inducing a greater
latency release for its onset. Progesterone induced an increase in the sexual receptivity of the females and did not
alter the biometric parameters like the body weight of the mothers and puppies, until weaning. Results gathered
here demonstrated that encapsulation of progesterone may be a promising alternative to the conventional
methods for synchronization of the estrous cycle of female animals in artificial insemination procedures.
Description
Keywords
SLN Nanoencapsulation Fixed-time artificial insemination Estrous cycle synchronization
Citation
Fogolari, Odinei; Leimann, Fernanda Vitória; Ineu, Rafael Porto; Rudy, Marcia; Ludwig, André Franco; Salles, Fernando Marques; Amorim, João Paulo de Arruda; Franci, Celso Rodrigues; Sagae, Sara Cristina; Sayer, Claudia; Araújo, Pedro Henrique Hermes de; Gonçalves, Odinei Hess (2023). Progesterone-loaded solid lipid nanoparticles for use in the regulation of the estrous cycle in female rats. Journal of Drug Delivery Science and Technology. ISSN 1773-2247. 88, p. 1-8
Publisher
Editions de Sante