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Effects of Cryphonectria parasitica infection by the hypovirus CHV1 on cellulase activity

2022Conference object Open access
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dc.contributor.authorTayechi, Yosri
dc.contributor.authorJorge, Lurdes
dc.contributor.authorCoelho, Valentim
dc.contributor.authorGouveia, Maria Eugénia
dc.date.accessioned2023-01-04T11:20:12Z
dc.date.available2023-01-04T11:20:12Z
dc.date.issued2022
dc.description.abstractCryphonectria parasitica is the causal agent of Castanea sativa chestnut blight, an economically important disease. Hypovirulence is due to the presence of a virus, the Cryphonectria hypovirus 1 (CHV1), that attenuates fungus pathogenicity by reducing the sporulation, and the enzymatic activity of pathogenesis-related enzymes. In several regions affected by C. parasitica, the release of C. parasitica hypovirulent strains on chestnut blight affected trees of C. sativa, has been used as a biological control. The objective of this work is to evaluate the effect of hypovirulence by CHV1 on the activity of cellulase, a pathogenesis-related enzyme, in different isolates of C. parasitica. For this, several virulent and hypovirulent (donors: RBB111, SR442, Serra05; and converted: Cast13RBB111, Cast13SR442, Curopos15SR442) isolates were grown in appropriate microbiological media, containing Carboxymethyl cellulose (CMC) and the enzyme activity was qualitatively evaluated. Contrary to expected, the hypovirulent donors (RBB111, SR442, Serra05) and converted strains showed higher cellulolytic activity than the virulent ones, evidencing higher ratio halo/growth (RHG). In the two converted Cast13 isogenic isolates, Cast13 RBB111 and Cast13 SR442 the obtained RHG were significantly different (p< 0.01), the last presented higher cellulolytic activity. In virulent strains, only Cp33PAR presented a small RHG, significantly different from the other virulent strains (p<0.05), without reaction.pt_PT
dc.description.sponsorshiptugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTayechi, Yosri; Jorge, Lurdes; Coelho, Valentim; Gouveia, Maria Eugénia (2022). Effects of Cryphonectria parasitica infection by the hypovirus CHV1 on cellulase activity. In IV Congresso Nacional das Escolas Superiores Agrárias: livro de resumos. Santarémpt_PT
dc.identifier.isbn978-989-53919-1-2
dc.identifier.urihttp://hdl.handle.net/10198/26275
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInstituto Politécnico de Santarémpt_PT
dc.relationMountain Research Center
dc.relationMountain Research Center
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHypovirulencept_PT
dc.subjectCryphonectria hypovirus 1 (CHV1)pt_PT
dc.subjectCellulasept_PT
dc.titleEffects of Cryphonectria parasitica infection by the hypovirus CHV1 on cellulase activitypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleMountain Research Center
oaire.awardTitleMountain Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00690%2F2020/PT
oaire.citation.conferencePlaceSantarémpt_PT
oaire.citation.endPage66pt_PT
oaire.citation.startPage66pt_PT
oaire.citation.titleIV Congresso Nacional das Escolas Superiores Agráriaspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCoelho
person.familyNameGouveia
person.givenNameValentim
person.givenNameMaria Eugénia
person.identifier.ciencia-id0618-49BC-EACE
person.identifier.orcid0000-0001-5829-7669
person.identifier.orcid0000-0002-2550-9108
person.identifier.scopus-author-id26533798200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication99a97d58-8b4c-4a49-9855-d334d568b9f3
relation.isAuthorOfPublication99e4701b-e3c2-4594-a37f-42bd49821d6e
relation.isAuthorOfPublication.latestForDiscovery99a97d58-8b4c-4a49-9855-d334d568b9f3
relation.isProjectOfPublication29718e93-4989-42bb-bcbc-4daff3870b25
relation.isProjectOfPublication0aac8939-28c2-46f4-ab6b-439dba7f9942
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