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Challenges in varroosis control: preliminary investigation of amitraz resistance in varroa destructor in Portugal

2024Conference object Open access
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dc.contributor.authorCosta, Maíra
dc.contributor.authorLopes, Ana
dc.contributor.authorYadró Garcia, Carlos A.
dc.contributor.authorGonçalves, Mariana Lousada
dc.contributor.authorCoelho, Liliana
dc.contributor.authorPires, Sancia
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.date.accessioned2024-10-07T14:07:42Z
dc.date.available2024-10-07T14:07:42Z
dc.date.issued2024
dc.description.abstractVarroosis is a disease caused by the ectoparasitic mite Varroa destructor, identified as one of the most significant global threats to the honey bee (Apis mellifera). The most effective control of this mite is through synthetic or organic acaricides. However, the excessive and repeated use of synthetic acaricides has led to the development of resistance. Amitraz is a synthetic pesticide commonly used in the control of V. destructor, but resistance to this compound has been observed. Previous studies observed a substitution of asparagine by serine at position 87 (N87S) of the Octβ2R gene, associated with amitraz resistance in France, and a substitution of tyrosine by histidine at position 215 (Y215H), associated with amitraz resistance in the USA. Building upon this knowledge, we aim to implement the first screening in Portugal for mutations associated with V. destructor resistance to amitraz. Unlike several European countries and the USA, Portugal lacks information regarding gene variation implicated in V. destructor resistance to amitraz, as well as allelic frequencies and their geographical distribution. To investigate the resistance mechanism, primers were developed to amplify the two known target regions of amitraz in V. destructor. DNA was extracted from individual female varroa mites using a commercial extraction kit, and the obtained DNA was PCR-amplified with the developed primers, followed by Sanger sequencing. With the knowledge obtained, we hope to assist beekeepers in selecting the most suitable acaricide to manage V. destructor in their apiaries and gain a deeper understanding of amitraz resistance in Portugal.pt_PT
dc.description.sponsorshipProject “MITE- Varroa e vírus transmitidos: Monitorização de mutações e desenvolvimento de ferramentas moleculares inovadoras” is funded by National Beekeeping Programme 2023-2027.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, Maíra; Lopes, Ana; Yadró Garcia, Carlos A.; Gonçalves, Mariana Lousada; Coelho, Liliana; Pires, Sancia; Pinto, M. Alice; Henriques, Dora (2024). Challenges in varroosis control: preliminary investigation of amitraz resistance in varroa destructor in Portugal. In 10th congress of Apidology. Tallinpt_PT
dc.identifier.urihttp://hdl.handle.net/10198/30329
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherEstonian University of Life Sciencespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectVarroa destructorpt_PT
dc.subjectAmitrazpt_PT
dc.subjectOctβ2Rpt_PT
dc.subjectPortugalpt_PT
dc.subjectResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technologypt_PT
dc.titleChallenges in varroosis control: preliminary investigation of amitraz resistance in varroa destructor in Portugalpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceTallinpt_PT
oaire.citation.endPage62pt_PT
oaire.citation.startPage62pt_PT
oaire.citation.title10th Congress of Apidologypt_PT
person.familyNameCosta
person.familyNameLopes
person.familyNameYadró García
person.familyNamePires
person.familyNamePinto
person.familyNameHenriques
person.givenNameMaíra
person.givenNameAna
person.givenNameCarlos A.
person.givenNameSancia
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idFB10-3DFA-D593
person.identifier.ciencia-idF014-6835-054D
person.identifier.ciencia-idF71F-B08E-EC39
person.identifier.ciencia-idCC12-1B38-26C8
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0002-1888-2262
person.identifier.orcid0000-0002-6916-3647
person.identifier.orcid0000-0001-8427-0179
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id53878422600
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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