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Chemometric evaluation of enzymatic hydrolysis in the production of fish protein hydrolysates with acetylcholinesterase inhibitory activity

2022Journal article Open access
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dc.contributor.authorMoreira, Thaysa Fernandes Moya
dc.contributor.authorPessoa, Luiz Gustavo Antunes
dc.contributor.authorSeixas, Flávio Augusto Vicente
dc.contributor.authorIneu, Rafael P.
dc.contributor.authorGonçalves, Odinei Hess
dc.contributor.authorLeimann, Fernanda Vitória
dc.contributor.authorRibeiro, Ricardo Pereira
dc.date.accessioned2021-08-31T10:26:20Z
dc.date.available2021-08-31T10:26:20Z
dc.date.issued2022
dc.description.abstractFish protein hydrolysates (FPH) obtained from industrial processing residues are sources of bioactive peptides. The enzymatic hydrolysis process is essential in obtaining specific bioactivities such as inhibition of the enzyme acetylcholinesterase (AChE). In this study the effect of different hydrolysis conditions on the properties of FPH to inhibit the enzyme acetylcholinesterase. A chemometric evaluation, based on a central composite rotatable design and principal component analysis, was applied to select hydrolysis conditions with best yield, degree of hydrolysis and acetylcholinesterase inhibition. Experimental design results for AChE inhibition were between 10.51 and 40.45% (20, 30 and 50 mg.mL-1 of FPH), and three hydrolysis conditions were selected based on PCA evaluation. The amino acids profile, FTIR and AChE inhibition kinetics were evaluated. Results showed a mixed type of inhibition behavior and, the docking molecular analyzes suggest that the inhibition AChE occurred due to the basic amino acids, mainly by arginine.pt_PT
dc.description.sponsorshipAuthors thank to CNPq (Chamada Universal– MCTI/CNPq N◦ 28/2018, Process 421541/2018-0) and Fundação Araucária (convênios 40/2016, 53/2019 and 039/2019) for the financial support. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Authors thank to Central Analítica Multiusu´ario da UTFPR Campo Mour˜ao (CAMulti-CM) by the analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMoreira, Thaysa Fernandes Moya; Pessoa, Luiz Gustavo Antunes; Seixas, Flavio Augusto Vicente; Ineu, Rafael Porto; Gonçalves, Odinei Hess; Leimann, Fernanda Vitória; Ribeiro, Ricardo Pereira (2022). Chemometric evaluation of enzymatic hydrolysis in the production of fish protein hydrolysates with acetylcholinesterase inhibitory activity. Food Chemistry. ISSN 0308-8146. 367, p. 1-9pt_PT
dc.identifier.doi10.1016/j.foodchem.2021.130728pt_PT
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/23839
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFish protein hydrolysatespt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.subjectExperimental designpt_PT
dc.subjectAcetylcholinesterasept_PT
dc.titleChemometric evaluation of enzymatic hydrolysis in the production of fish protein hydrolysates with acetylcholinesterase inhibitory activitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage130728pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume367pt_PT
person.familyNameGonçalves
person.familyNameLeimann
person.givenNameOdinei Hess
person.givenNameFernanda Vitória
person.identifier.ciencia-id8F10-A04D-FC0D
person.identifier.orcid0000-0002-9528-8187
person.identifier.orcid0000-0001-6230-9597
person.identifier.scopus-author-id9335958400
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication84c8eea0-77bb-4c18-b9d8-46f865980706
relation.isAuthorOfPublicationb1102a0b-38dc-4caa-baed-ad52ccd9b230
relation.isAuthorOfPublication.latestForDiscovery84c8eea0-77bb-4c18-b9d8-46f865980706

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