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Inactivation of Staphylococcus aureus by antimicrobial photodynamic therapy using 1,9-Dimethyl-Methylene Blue: in vitro and in vivo studies

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datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
datacite.subject.fosCiências Médicas::Biotecnologia Médica
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorBrito Júnior, Anildo Alves de
dc.contributor.authorCrugeira, Pedro
dc.contributor.authorBarbosa, Andressa Vollono
dc.contributor.authorCosta, Wellington Luis Reis
dc.contributor.authorCangussu, Maria Cristina Teixeira
dc.contributor.authorOliveira, Susana Carla Pires Sampaio de
dc.contributor.authorPinheiro, Antônio Luiz Barbosa
dc.contributor.authorMelo, Amanda Inês Vieira de
dc.contributor.authorPinheiro, Antônio Luiz Barbosa
dc.contributor.authorAzevedo, Juliana Monteiro
dc.date.accessioned2025-03-14T16:38:38Z
dc.date.available2025-03-14T16:38:38Z
dc.date.issued2024
dc.description.abstractThe efficiency of antibiotics in terms of their bacterial inhibition is well known. However, studies show that its overuse, underuse, and misuse induce antimicrobial resistance, promoting the need to work with alternative methods. In this sense, antimicrobial photodynamic therapy (aPDT) is a promising selective method demonstrating excellent response. This study aimed to evaluate the antimicrobial action promoted in Staphylococcus aureus using 1,9 dimethyl methylene blue dye (DMMB) combined with red LED (λ 630 ± 20 nm, CW, 125 mW, 12 J/ cm², 192 s) in planktonic culture and rats skin wounds contaminated with staphylococcal bacteria. The experimental in vitro and in vivo groups were Control, LED, DMMB, and LED + DMMB; after aPDT, the triplicate samples for each dilution were incubated for 24 h, and the number of bacteria was determined by counting the colony-forming units, and the logarithm (CFU/mL log). Based on in vitro data obtained, the LED + DMMB group, when compared to the Control, showed a reduction in microbial load of 99.943% (p < 0.0001), with decimal reduction (RD = 3). Whereas in vivo results, the same comparing groups demonstrated a reduc- tion in microbial load, reaching 99.994% (RD = 4). In this research, the aPDT was a unique treatment, and it is possible to repeat it to obtain higher microbial reduction, providing an alternative therapeutic that can be clinically validated to combat infections caused by S. aureus.por
dc.description.sponsorshipThe authors would like to thank the Coorde- nação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES (Coordination for the Improvement of Higher Education Personnel) for the master’s scholarship for Anildo A. de B. Júnior (process nº 88887.670810/2022-00) and the Programa Institucional de Bolsas de Iniciação Científica e Tecnológica/Conselho Nacional de Desen- volvimento Científico e Tecnológico - PIBITI/CNPq (Institutional Programme for Scientific and Technological Initiation Scholarships/ National Council for Scientific and Technological Development) for the scholarship for Amanda I. Vieira Mello (process nº 162148/2023- 6). V. de Mello (process nº 162147/2023-6). OleaChain project “Competences for sustainability and innovation in the value chain of traditional olive groves in the interior north of Portugal” (NORTE- 06-3559-FSE-000188) for Pedro J. L. Crugeira. Author A. L. B. Pin- heiro would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the research grant (304279/2018- 8). Open access funding provided by FCT|FCCN (b-on).
dc.identifier.citationBrito Júnior, Anildo Alves de; Crugeira, Pedro; Barbosa, Andressa Vollono; Costa, Wellington Luis Reis; Cangussu, Maria Cristina Teixeira; Oliveira, Susana Carla Pires Sampaio de; Pinheiro, Antônio Luiz Barbosa; Melo, Amanda Inês Vieira de; Pinheiro, Antônio Luiz Barbosa; Azevedo, Juliana Monteiro (2024). Inactivation of Staphylococcus aureus by antimicrobial photodynamic therapy using 1,9-Dimethyl-Methylene Blue: in vitro and in vivo studies. Lasers in Medical Science. ISSN 0006-3363. 40:1, p. 1-8
dc.identifier.doi10.1007/s10103-025-04362-6
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttp://hdl.handle.net/10198/34356
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Nature
dc.relation.ispartofBiology of Reproduction
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectStaphylococcus aureus
dc.subjectPhotochemotherapy
dc.subjectPhotosensitizing drugs
dc.subjectLED
dc.titleInactivation of Staphylococcus aureus by antimicrobial photodynamic therapy using 1,9-Dimethyl-Methylene Blue: in vitro and in vivo studiespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage8
oaire.citation.issue1
oaire.citation.startPage1
oaire.citation.titleLasers in Medical Science
oaire.citation.volume40
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCrugeira
person.givenNamePedro
person.identifier.ciencia-id8F17-E60C-A45D
person.identifier.orcid0000-0002-7084-3711
relation.isAuthorOfPublicationce777646-e21d-4491-a8a8-345efc9517a1
relation.isAuthorOfPublication.latestForDiscoveryce777646-e21d-4491-a8a8-345efc9517a1

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