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Development and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruits

2002Artigo científico Acesso aberto
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dc.contributor.authorVinha, Ana F.
dc.contributor.authorSilva, Branca M.
dc.contributor.authorAndrade, Paula B.
dc.contributor.authorSeabra, Rosa M.
dc.contributor.authorPereira, J.A.
dc.contributor.authorOliveira, Beatriz
dc.date.accessioned2008-09-03T09:33:47Z
dc.date.available2008-09-03T09:33:47Z
dc.date.issued2002
dc.description.abstractA new HPLC/DAD methodology for separating nine phenolic compounds is described. This methodology is applied to the definition of qualitative and quantitative profiles of three Portuguese olive fruit cultivars (Cobrançosa, Madural and Verdeal). Two different extraction methods were needed for the complete definition of their profiles, one of them including a Sep-pack C18 cleaning step. The chromatographic separation was achieved using a Spherisorb ODS2 (25.0 × 0.46 cm; 5 μm, particle size) column. The solvent system used was a gradient of water-formic acid (19:1) and methanol, with a flow rate of 0.9 mL/min. The detection limit values for phenolic compounds were between 0.04 and 4.32 μg/mL and the method was precise. As a general rule, the recovery values were high. This technique can also be useful in the discrimination of Portuguese olive fruit cultivarsen
dc.identifier.citationVinha, A.F.; Silva, B.M.; Andrade, P.B.; Seabra, R.M.; Pereira, J.A.; Oliveira, M.B.P.P. (2002). Development and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruits. Journal Liquid Chromatography & Related Technologies. ISSN 1082-6076. 25:1, p. 51-160en
dc.identifier.doi10.1081/JLC-100108546
dc.identifier.issn1082-6076
dc.identifier.urihttp://hdl.handle.net/10198/723
dc.language.isoengen
dc.peerreviewedyes
dc.publisherMarcel Dekkeren
dc.subjectHigh performance liquid chromatographyen
dc.subjectDiodesen
dc.subjectPhenolsen
dc.titleDevelopment and evaluation of an HPLC/DAD method for the analysis of phenolic compounds from olive fruitsen
dc.typejournal article
dspace.entity.typePublication
person.familyNamePereira
person.givenNameJosé Alberto
person.identifier.ciencia-id611F-80B2-A7C1
person.identifier.orcid0000-0002-2260-0600
person.identifier.ridL-6798-2014
person.identifier.scopus-author-id57204366348
rcaap.rightsopenAccess
rcaap.typearticleen
relation.isAuthorOfPublication7932162e-a2da-4913-b00d-17babbe51857
relation.isAuthorOfPublication.latestForDiscovery7932162e-a2da-4913-b00d-17babbe51857

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