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A comparative study of image processing methods for the assessment of the red blood cells deformability in a microfluidic device

2018Conference paper Open access
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dc.contributor.authorFaustino, Vera
dc.contributor.authorCatarino, Susana
dc.contributor.authorPinho, Diana
dc.contributor.authorMinas, Graça
dc.contributor.authorLima, Rui A.
dc.date.accessioned2018-01-31T10:00:00Z
dc.date.accessioned2018-02-01T09:37:51Z
dc.date.available2018-01-31T10:00:00Z
dc.date.available2018-02-01T09:37:51Z
dc.date.issued2018
dc.description.abstractRed blood cells (RBCs) deformability is a high relevant mechanical property, whose variations are associated with some diseases, such as diabetes and malaria. Therefore, the present study aims to compare different image processing methods for assessing the RBCs deformability in a continuous flow, measured in a polydimethylsiloxane (PDMS) microchannel composed by 15 μm spacing inner pillars. The images were acquired with a high speed camera and analyzed with ImageJ software for tracking and measuring the RBCs deformation index (DI). Additionally, to understand the performance of the software, it was performed a comparison between different image processing tools provided by ImageJ and the best methods for the deformation measurements were selected, considering the measured RBCs number and their DIs. The results show that those image methods significantly affect the number of measured RBCs and their DIs and, therefore, the studies focused on the deformability measurements need to take into account the effect of the image processing methods for avoiding loss of relevant information in the images.en_EN
dc.description.sponsorshipThis work was supported by FCT with the reference project UID/EEA/ 04436/2013, by FEDER funds through the COMPETE 2020 – Programa Operacional Competitividade e Internacionalização (POCI) with the reference project POCI-01- 0145-FEDER- 006941. V. Faustino, S.O. Catarino and D. Pinho thank, respectively, the FCT for the grants SFRH/BD/ 99696/2014, SFRH/BPD/108889/2015 and SFRH/BD/89077/2012, supported by national funds from Ministérios da Ciência, Tecnologia e Ensino Superior and by FSE through the POCH - Programa Operacional Capital Humano. The authors also acknowledge the financial support provided by the project Nos. UID/EMS/00532/2013 and UID/EMS/04077/2013.
dc.description.versioninfo:eu-repo/semantics/publishedVersionen_EN
dc.identifier.citationFaustino, Vera; Catarino, Susana O.; Pinho, Diana; Minas, Graça; Lima, Rui (2018). A comparative study of image processing methods for the assessment of the red blood cells deformability in a microfluidic device. Lecture Notes in Computational Vision and Biomechanics. ISSN 2212-9391. 27, p. 923-929en_EN
dc.identifier.doi10.1007/978-3-319-68195-5_100en_EN
dc.identifier.issn2212-9391
dc.identifier.urihttp://hdl.handle.net/10198/15389
dc.language.isoeng
dc.peerreviewedyesen_EN
dc.relationMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
dc.relationMultifunctional lab-on-a-chip for blood properties detection with integrated PVDF acoustic transducers: application to malaria biomarkers
dc.subjectDeformabilityen_EN
dc.subjectDeformation indexen_EN
dc.subjectImage processingen_EN
dc.subjectMicrofluidicsen_EN
dc.subjectRed blood cellsen_EN
dc.titleA comparative study of image processing methods for the assessment of the red blood cells deformability in a microfluidic deviceen_EN
dc.typeconference paper
dspace.entity.typePublication
oaire.awardTitleMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methods
oaire.awardTitleMultifunctional lab-on-a-chip for blood properties detection with integrated PVDF acoustic transducers: application to malaria biomarkers
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F99696%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F108889%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89077%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEMS%2F00532%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEMS%2F04077%2F2013/PT
oaire.fundingStreamFARH
oaire.fundingStreamSFRH
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNamePinho
person.familyNameLima
person.givenNameDiana
person.givenNameRui A.
person.identifier.ciencia-id5214-ED0C-35E8
person.identifier.ciencia-idEE12-C3FB-349D
person.identifier.orcid0000-0002-3884-6496
person.identifier.orcid0000-0003-3428-637X
person.identifier.ridH-5157-2016
person.identifier.scopus-author-id18437397800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccessen_EN
rcaap.typeconferenceObjecten_EN
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