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Recovery of bioactive polyphenols from Aloe vera (Aloe barbadensis Mill.) rind using binary mixtures of propylene glycol and water

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dc.contributor.authorFerreira, Olga
dc.contributor.authorAñibarro-Ortega, Mikel
dc.contributor.authorPinela, José
dc.contributor.authorBarros, Lillian
dc.date.accessioned2021-10-28T09:37:08Z
dc.date.available2021-10-28T09:37:08Z
dc.date.issued2019
dc.description.abstractAloe vera (Aloe barbadensis Mill.) is a succulent plant species cultivated worldwide for its jelly-like parenchyma that is used by cosmetic, pharmaceutical and nutraceutical industries. This gel fillet is found protected by a thick layer (green rind) that constitutes 20-30% of the whole leaf weight [1], which is often discarded as waste. However, this biowaste can be valorised as a source of high added-value compounds [2]. Today, the principles of green chemistry have been introduced into extraction schemes with the aim of making processes more sustainable. Some studies have also shown that polyols in aqueous solution may constitute a very suitable extraction medium for polyphenol recovery [3]. Therefore, this study was carried out to characterize the phenolic profile of Aloe vera rind, evaluate antioxidant properties, and investigate the suitability of propylene glycol-water mixtures for extracting these bioactives. The leaf rind was separated from the fillet gel parenchyma, freeze-dried and reduced to a fine powder. First, a solid-liquid extraction was performed with ethanol/water (80:20, v/v) to obtain a combined extract, from which the phenolic profile was characterized by HPLC-DAD-ESI/MS and the antioxidant activity was evaluated by the in vitro cell-based assays of TBARS (thiobarbituric acid reactive substances) and OxHLIA (oxidative haemolysis inhibition assay) [4]. Afterwards, binary mixtures of propylene glycol (propane-1,2-diol)-water (from 0 to 95 %, w/w) were used in extractions performed at 50 oC for 60 min, whose efficiency was monitored based on the total phenolic and flavonoid contents, measured by colorimetric methods [5]. Different phenolic compounds were identified in the Aloe vera rind extract, including chromones (aloesin A and B), anthrones (aloin A and B), and flavones (luteolin and apigenin glucoside derivatives). Aloesin and aloin (Figures 1 and 2) are recognized for their skin regeneration (wound healing) and laxative effects, respectively, being among the most important physiologically active compounds found in Aloe [6,7]. The combined extract also had interesting antioxidant properties, being particularly effective in protecting erythrocytes from the free radical-induced oxidative damage, with an IC50 value close to that of the positive control, trolox. Regarding the effect of the extraction solvents on the recovery of total phenolics and flavonoids, it was found that intermediate propylene glycol-water mixtures lead to higher amounts than when used alone. Thus, this study showed that Aloe vera rind can be used as an interesting source of bioactive compounds and that propylene glycol in aqueous solution may improve its extraction.pt_PT
dc.description.sponsorshipAcknowledgements: To the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013) and the research contracts of J. Pinela (Project AllNatt, POCI-01-0145-FEDER-030463) and L. Barros. To POCI-01-0145-FEDER-006984 (LA LSRE-LCM), funded by ERDF, through POCI-COMPETE2020 and FCT. This work was funded by the European Regional Development Fund (ERDF) through the Competitiveness and Internationalization Operational Program (POCI) and national funding through FCT, within the scope of Project POCI-01-0145-FEDER-030463: AllNat. To FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E. Also to the company "Courela do Alentejo" for the plant samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, Olga; Añibarro-Ortega, Mikel; Pinela, José; Barros, Lillian (2019). Recovery of bioactive polyphenols from Aloe vera (Aloe barbadensis Mill.) rind using binary mixtures of propylene glycol and water. In ISCG International Symposium on Green Chemistry. La Rochellept_PT
dc.identifier.urihttp://hdl.handle.net/10198/24113
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.titleRecovery of bioactive polyphenols from Aloe vera (Aloe barbadensis Mill.) rind using binary mixtures of propylene glycol and waterpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLa Rochelle, Françapt_PT
oaire.citation.titleInternational Symposium on Green Chemistrypt_PT
person.familyNameFerreira
person.familyNameAñibarro-Ortega
person.familyNamePinela
person.familyNameBarros
person.givenNameOlga
person.givenNameMikel
person.givenNameJosé
person.givenNameLillian
person.identifier469085
person.identifier.ciencia-id9F1D-C75A-4A54
person.identifier.ciencia-idC61D-DD88-73C7
person.identifier.ciencia-id771C-2B43-B108
person.identifier.ciencia-id9616-35CB-D001
person.identifier.orcid0000-0001-8414-3479
person.identifier.orcid0000-0002-2835-5079
person.identifier.orcid0000-0001-7523-1637
person.identifier.orcid0000-0002-9050-5189
person.identifier.ridM-4567-2013
person.identifier.ridB-4466-2014
person.identifier.ridJ-3600-2013
person.identifier.scopus-author-id57203083219
person.identifier.scopus-author-id54392272800
person.identifier.scopus-author-id35236343600
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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