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Suillus collinitus methanolic extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line

2012Journal article Open access
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dc.contributor.authorVaz, Josiana A.
dc.contributor.authorFerreira, Isabel C.F.R.
dc.contributor.authorTavares, Catarina
dc.contributor.authorAlmeida, Gabriela M.
dc.contributor.authorMartins, Anabela
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2013-01-03T18:30:08Z
dc.date.available2013-01-03T18:30:08Z
dc.date.issued2012
dc.description.abstractIn the present work, methanolic, ethanolic and boiled water extracts of Suillus collinitus were chemically characterized and further submitted to evaluation of their bioactive properties (antioxidant potential and cytotoxic activity in tumor cell lines). Phenolic acids and sugars were chromatographically identified and quantified in the methanolic and boiled water extracts, respectively. S. collinitus ethanolic extract gave the highest antioxidant activity. Nevertheless, regarding cell growth inhibition, the methanolic extract was the most potent one, particularly in MCF-7 cells (GI50 25.2±0.2µg/ml). Moreover, the GI50 concentration induced a G1 cell cycle arrest, with a concomitant decrease in the percentage of cells in the S phase. Furthermore, it caused an increase in the percentage of apoptotic cells, from 6.0±0.2% in untreated cells, to 15.3±2.0% in cells treated with the GI50 concentration and to 16.3±2.0% in cells treated with 2×GI50 concentration. In addition, 48 h treatment with the GI50 concentration caused a strong increase in the levels of p53, p21, and cleaved PARP, together with a decrease in Bcl-2 and XIAP. Results indicate that Suillus collinitus may be a promising source of bioactive compounds. Particularly, its methanolic extract appears to have a p53-mediated effect on the normal cell cycle distribution and apoptosis induction in a human breast tumor cell line.por
dc.identifier.citationVaz, Josiana A.; Ferreira, Isabel C.F.R.; Tavares, Catarina; Almeida, Gabriela M.; Martins, Anabela; Vasconcelos, M. Helena (2012). Suillus collinitus methanolic extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line. Food Chemistry. ISSN 0308-8146. 135:2, p. 596–602por
dc.identifier.doi10.1016/j.foodchem.2012.04.127
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10198/7811
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBD/43653/2008
dc.relationPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
dc.relationStrategic Project - UI 690 - 2011-2012
dc.subjectWild mushroompor
dc.subjectAntioxidant potentialpor
dc.subjectBreast cancerpor
dc.subjectApoptosis inductionpor
dc.subjectp53-mediated effectpor
dc.titleSuillus collinitus methanolic extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell linepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines
oaire.awardTitleStrategic Project - UI 690 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F110062%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0690%2F2011/PT
oaire.citation.endPage602por
oaire.citation.startPage596por
oaire.citation.titleFood Chemistrypor
oaire.citation.volume135por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVaz
person.familyNameFerreira
person.familyNameMartins
person.givenNameJosiana A.
person.givenNameIsabel C.F.R.
person.givenNameAnabela
person.identifier144781
person.identifier.ciencia-id171B-88BA-64F5
person.identifier.ciencia-id9418-CF95-9919
person.identifier.ciencia-id7B18-A810-6B93
person.identifier.orcid0000-0002-6989-1169
person.identifier.orcid0000-0003-4910-4882
person.identifier.orcid0000-0001-6218-4413
person.identifier.ridE-8500-2013
person.identifier.ridG-5488-2013
person.identifier.scopus-author-id16305323200
person.identifier.scopus-author-id36868826600
person.identifier.scopus-author-id7203013518
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication1c1a2cb9-5a36-47ba-bca0-ffcbef28df18
relation.isAuthorOfPublicationbd0d1537-2e03-41fb-b27a-140af9c35db8
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