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Honeybee gut microbiota is an imperative endpoint for pesticide risk assessment

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dc.contributor.authorRosa-Fontana, Annelise
dc.contributor.authorAguado-López, Daniel
dc.contributor.authorUriel Clara, Jabal
dc.contributor.authorMartín-Hernández, Raquel
dc.contributor.authorHiges, Mariano
dc.contributor.authorPinto, M. Alice
dc.contributor.authorHenriques, Dora
dc.contributor.authorTosi, Simone
dc.contributor.authorRodríguez Gomez, Juan Miguel
dc.date.accessioned2024-11-05T11:33:29Z
dc.date.available2024-11-05T11:33:29Z
dc.date.issued2024
dc.description.abstractIn nature, honeybee workers acquire their stable gut microbial community by the 7th day post-emergence, with older bees transmitting microorganisms to younger bees in the comb, thereby establishing natural microbial diversity. In contrast, younger caged bees sampled for laboratory trials (OECD Guideline No. 245) are in contact with older bees for only a few hours. Newly emerged bees harbor minimal to no bacteria, potentially resulting in lower diversity, richness, and bacterial loads in their gut. However, this method best simulates the natural state within a controlled environment.Existing studies have modified standardized protocols to simulate the microbiota present in the honeybee digestive tract within the hive environment. A common approach involves diluting the gut contents of forager bees and incorporating this into the diet of caged bees. In our trials, we strictly adhered to OECD Guideline No. 245 (Chronic Oral Toxicity Test; 10-Day Feeding), exposing newly emerged Apis mellifera carnica workers to a single concentration of the insecticide flupyradifurone (FPF, 36 ppm). The standard reference dimethoate (1 ppm) and control groups (pure food and food + acetone) were also included. DNA was extracted individually from the bee abdomens, and full-length 16S rRNA amplicon metagenomics were sequenced using PacBio Sequel II (HiFi/CCS mode). The absolute abundance of four bacterial genera comprising the core honeybee microbiota revealed a Lactobacillus-dominated gut in both treated and untreated bees. Treated bees exhibited a twofold increase in the bacterial load of Snodgrassella, contrasting with a 50% reduction in Bifidobacterium and the complete absence of Gilliamella compared to untreated bees. Our findings demonstrate that FPF significantly disrupts the honeybee gut microbiota. This study presents, for the first time, the composition of the gut microbiota in honeybees strictly subjected to the OECD guideline without modifications or adaptations. Results from OECD-based tests already meet reliability requirements for risk assessments. Therefore, following OECD standards strictly illuminate three distinct advantages: (1) streamlining the process leading to a ring test, (2) reducing variations introduced by external factors potentially brought into hives by foraging bees, and (3) reducing bacterial diversity in lab-tested bees, thereby facilitating the establishment of acceptable fluctuations in microbiota composition. We have developed a new approach, overlooked in risk assessments studies so far, to assess the impact of pesticides on bee health. We propose adopting this approach as a new endpoint in pesticide risk assessments. Specifically, we advocate for the inclusion of honeybee gut microbiota dysbiosis as a sublethal effect in the first screening step of risk assessments, and as a key parameter to assess pollinator's health. We will present a summary of the most relevant bacteria for bee health, alongside fluctuations in the microbiota and diversity indices. Additionally, we will provide recommendations on the most suitable indicators for assessing gut microbiota dysbiosis.pt_PT
dc.description.sponsorshipThis project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie-Curie grant agreement No 847635pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRosa-Fontana, Annelise; Aguado-López, Daniel; Uriel Clara, Jabal; Martín-Hernández, Raquel; Higes, Mariano; Pinto, M. Alice; Henriques, Dora; Tosi, Simone; Rodríguez Gomez, Juan Miguel (2024). Honeybee gut microbiota is an imperative endpoint for pesticide risk assessment. In 16th International Symposium Hazards of Pesticides to Bees. Sevilla, p. 51-53pt_PT
dc.identifier.urihttp://hdl.handle.net/10198/30500
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInternational Commission for Plant-Pollinator Relationshipspt_PT
dc.relationUNA Europa, an alliance of universities FOR the emergence of talent and the development of research CAREERs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGut microbiotapt_PT
dc.subjectHoney beept_PT
dc.subjectPesticidept_PT
dc.subjectRisk assessmentpt_PT
dc.titleHoneybee gut microbiota is an imperative endpoint for pesticide risk assessmentpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleUNA Europa, an alliance of universities FOR the emergence of talent and the development of research CAREERs
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/847635/EU
oaire.citation.conferencePlaceSevilhapt_PT
oaire.citation.title16th International Symposium Hazards of Pesticides to Beespt_PT
oaire.fundingStreamH2020
person.familyNamePinto
person.familyNameHenriques
person.givenNameM. Alice
person.givenNameDora
person.identifier.ciencia-idF814-A1D0-8318
person.identifier.ciencia-id291F-986F-07DA
person.identifier.orcid0000-0001-9663-8399
person.identifier.orcid0000-0001-7530-682X
person.identifier.scopus-author-id8085507800
person.identifier.scopus-author-id55761737300
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication0667fe04-7078-483d-9198-56d167b19bc5
relation.isAuthorOfPublicationd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isAuthorOfPublication.latestForDiscoveryd2abd09f-a90c-4cfb-9a60-7fc32f56184d
relation.isProjectOfPublicationd5a8bf8c-f056-4c70-887c-fa3b9697f550
relation.isProjectOfPublication.latestForDiscoveryd5a8bf8c-f056-4c70-887c-fa3b9697f550

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