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Advisor(s)
Abstract(s)
Single nucleotide polymorphisms (SNPs) have a great potential in genetic identification and introgression studies of honey bees (Apis mellifera). To achieve SNPs full power in genetic analysis, they must be screened in a rapid, accurate and cost-effective manner. Matrix-assisted laser desorption-ionization–time-of-flight (MALDI–TOF) mass spectrometry is a promising tool for the high-throughput screening of SNPs at an affordable cost in the post-genome sequencing era. In this study, a reduced SNP panel has been validated for Iberian honey bees (A. m. iberiensis) and a pooling strategy is presented for allele frequencies determination by using MALDI-TOF technology. The reduced SNP panel contains 127 most ancestry-informative loci design in the dark honey bee (A. m. mellifera). We demonstrate the utility of this methodology in samples of Iberian honey bees (A. m. iberiensis) selected for its pathogen tolerance and in genomic DNA pools. The described method reduces costs and time and enables genotyping of up to thousands of honey bee samples by taking advantage of the high-throughput MALDI-TOF technology.
Description
Keywords
SNP assays MALDI-TOF genotyping Honeybee Apis mellifera
Citation
Muñoz, Irene; Martín-Hernández, Raquel; Higes, Mariano; De la Rúa, Pilar; Pinto, M. Alice (2017). SNP assay validation in honey bee populations and allele frequency determination in DNA pools by MALDI-TOF MS. In XVI Congress of the European Society for Evolutionary Biology. Groningen