Repository logo
 
Publication

CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing

2022Journal article Open access
Simple item page
dc.contributor.authorFerreira, Patrick
dc.contributor.authorChoupina, Altino
dc.date.accessioned2022-07-18T13:38:55Z
dc.date.available2022-07-18T13:38:55Z
dc.date.issued2022
dc.description.abstractIn recent years, the number of tools and techniques that enable genetic material to be added, removed or altered at specifc locations in the genome has increased signifcantly. The objective is to know the structure of genomes, the function of genes and improve gene therapy. In this work we intend to explain the functioning of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palin dromic Repeats/CRISPR associated protein 9) and the advantages that this technique may have compared to previously developed techniques, such as RNA interference (RNAi), Zinc Finger Nucleases (ZFNs) and transcription activator-like efector nucleases (TALENs) in gene and genome editing. We will start with the story of the discovery, then its biological function in the adaptive immune system of bacteria against bacteriophage attack, and ending with a description of the mechanism of action and its use in gene editing. We will also discuss other Cas enzymes with great potential for use in genome editing as an alternative to Cas9. CRISPR/Cas9 is a simple, inexpensive, and efective technique for gene editing with multiple applications from the development of functional genomics and epigenetics. This technique will, in the near future, have great applications in the development of cell models for use in medical and pharmaceutical processes, in targeted therapy, and improvement of agricultural and environmental species.pt_PT
dc.description.sponsorshipThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFerreira, Patrick; Choupina, Altino (2022). CRISPR/Cas9 a simple, inexpensive and effective technique for gene editing. Molecular Biology Reports. ISSN 0301-4851. 49:7, p. 7079-7086pt_PT
dc.identifier.doi10.1007/s11033-022-07442-wpt_PT
dc.identifier.issn0301-4851
dc.identifier.urihttp://hdl.handle.net/10198/25680
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relationMountain Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCRISPRpt_PT
dc.subjectCas9pt_PT
dc.subjectEditing genespt_PT
dc.subjectGenome engineeringpt_PT
dc.titleCRISPR/Cas9 a simple, inexpensive and effective technique for gene editingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMountain Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2019/PT
oaire.citation.conferencePlaceSpringer Nature Switzerlandpt_PT
oaire.citation.endPage8pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMolecular Biology Reportspt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameChoupina
person.givenNameAltino
person.identifier587972
person.identifier.ciencia-id1A14-77FC-9656
person.identifier.orcid0000-0002-3956-9398
person.identifier.ridL-5382-2014
person.identifier.scopus-author-id14051602500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isAuthorOfPublication.latestForDiscovery314b895b-a5e2-4a92-9bc6-dd708c819be4
relation.isProjectOfPublicationd6683ba1-d253-48e4-968e-35106ce7b750
relation.isProjectOfPublication.latestForDiscoveryd6683ba1-d253-48e4-968e-35106ce7b750

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
CRISPRCas9ASimpleInexpensiveAn.pdf
Size:
1.01 MB
Format:
Adobe Portable Document Format
Download

Collections

CIMO - Artigos em Revistas Indexados à WoS/Scopus